Literature DB >> 1238083

Purification and properties of rat brain pyruvate carboxylase.

D E Mahan, I K Mushahwar, R E Koeppe.   

Abstract

Rat brain pyruvate carboxylase was purified 2000-fold and some of its properties and kinetic parameters were investigated. The use of (NH4)2SO4 gradient solubilization on a Celite column and precipitation with polyethylene glycol permitted purification to an estimated 20% purity. Except for a few subtle kinetic differences this enzyme is indistinguishable from rat liver pyruvate carboxylase.

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Year:  1975        PMID： 1238083      PMCID： PMC1165183          DOI： 10.1042/bj1450025

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  43 in total

1.  QUANTITATIVE ASPECTS OF CO2 FIXATION IN MAMMALIAN BRAIN IN VIVO.

Authors:  H WAELSCH; H W BERL; C A ROSSI; D D CLARKE; D P PURPURA
Journal:  J Neurochem       Date:  1964-10       Impact factor: 5.372

2.  Statistical estimations in enzyme kinetics.

Authors:  G N WILKINSON
Journal:  Biochem J       Date:  1961-08       Impact factor: 3.857

3.  A method for determining the sedimentation behavior of enzymes: application to protein mixtures.

Authors:  R G MARTIN; B N AMES
Journal:  J Biol Chem       Date:  1961-05       Impact factor: 5.157

4.  The enzymic hydrolysis of adenosine triphosphate by liver mitochondria. I. Activities at different pH values.

Authors:  D K MYERS; E C SLATER
Journal:  Biochem J       Date:  1957-12       Impact factor: 3.857

5.  The incorporation in vitro of C14 into amino acids of control and virus-infected mouse brain.

Authors:  K MOLDAVE; R J WINZLER; H E PEARSON
Journal:  J Biol Chem       Date:  1953-01       Impact factor: 5.157

6.  Possible regulatory factors for pyruvate carboxylase with particular reference to enzyme from chicken liver.

Authors:  M C Scrutton
Journal:  Metabolism       Date:  1971-02       Impact factor: 8.694

7.  A kinetic analysis of coupled enzyme assays.

Authors:  W R McClure
Journal:  Biochemistry       Date:  1969-07       Impact factor: 3.162

8.  The effect of thiamine deficiency on the acetylcoenzyme A and acetylcholine levels in the rat brain.

Authors:  C P Heinrich; H Stadler; H Weiser
Journal:  J Neurochem       Date:  1973-11       Impact factor: 5.372

9.  Pyruvate carboxylase from rat liver: catalytic properties in the absence, and at low concentrations, of acetyl-CoA.

Authors:  M C Scrutton; M D White
Journal:  Biochem Biophys Res Commun       Date:  1972-07-11       Impact factor: 3.575

10.  Pyruvate carboxylase activity of mammalian brain cortex.

Authors:  R A Felicioli; F Gabrielli; C A Rossi
Journal:  Life Sci       Date:  1967-01-15       Impact factor: 5.037
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  1 in total

1.  Inhibitors of Pyruvate Carboxylase.

Authors:  Tonya N Zeczycki; Martin St Maurice; Paul V Attwood
Journal:  Open Enzym Inhib J       Date:  2010
  1 in total

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