Literature DB >> 12379057

The value of tissue imprint hybridization for rapid detection of infectious bursal disease virus from field outbreaks.

M Parthiban1, V Thiagarajan.   

Abstract

The use of a peroxidase labelled PCR generated probe followed by enhanced chemiluminescence hybridization assay detected infectious bursal disease virus directly from bursal imprints on a nylon membrane. Tissue imprint hybridization proved to be a simple, rapid and safe means of detecting IBD virus for screening large numbers of field samples. The PCR generated probe was highly specific for IBD virus and did not hybridize with cellular nucleic acids in control imprints. Tissue imprint hybridization was found to be a more sensitive method than conventional antigen detection assays.

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Year:  2002        PMID： 12379057     DOI： 10.1023/a:1020040125341

Source DB:  PubMed          Journal:  Trop Anim Health Prod        ISSN： 0049-4747            Impact factor:   1.559


  13 in total

1.  Highly sensitive and specific non-radioactive biotinylated probes for dot-blot, Southern and colony hybridizations.

Authors:  M Eweida; T L Sit; S Sira; M G AbouHaidar
Journal:  J Virol Methods       Date:  1989-10       Impact factor: 2.014

2.  An improved dot ELISA to detect fowl adenovirus type-1 antigen.

Authors:  N K Maiti; S S Saini; R Singh; M S Oberoi; S N Sharma
Journal:  Comp Immunol Microbiol Infect Dis       Date:  1993-07       Impact factor: 2.268

3.  Immunodiffusion reaction to avian infectious bursal virus.

Authors:  K Hirai; S Shimakura; M Hirose
Journal:  Avian Dis       Date:  1972 Jul-Sep       Impact factor: 1.577

4.  Comparison of the dot blot hybridization assay with antigen detection assays for the diagnosis of infectious bursal disease virus infections.

Authors:  K S Henderson; D J Jackwood
Journal:  Avian Dis       Date:  1990 Jul-Sep       Impact factor: 1.577

5.  Sequence comparisons of a highly virulent infectious bursal disease virus prevalent in Japan.

Authors:  Z Lin; A Kato; Y Otaki; T Nakamura; E Sasmaz; S Ueda
Journal:  Avian Dis       Date:  1993 Apr-Jun       Impact factor: 1.577

6.  Tissue-print hybridization using a non-radioactive probe for the detection of infectious bursal disease virus.

Authors:  T L Hathcock; J J Giambrone
Journal:  Avian Dis       Date:  1992 Apr-Jun       Impact factor: 1.577

7.  Rapid and sensitive colorimetric method for visualizing biotin-labeled DNA probes hybridized to DNA or RNA immobilized on nitrocellulose: Bio-blots.

Authors:  J J Leary; D J Brigati; D C Ward
Journal:  Proc Natl Acad Sci U S A       Date:  1983-07       Impact factor: 11.205

8.  Detection of genetic variations in serotype I isolates of infectious bursal disease virus using polymerase chain reaction and restriction endonuclease analysis.

Authors:  H J Liu; J J Giambrone; T Dormitorio
Journal:  J Virol Methods       Date:  1994-07       Impact factor: 2.014

9.  Detection of infectious bursal disease viruses using in situ hybridization and non-radioactive probes.

Authors:  D J Jackwood; D E Swayne; R J Fisk
Journal:  Avian Dis       Date:  1992 Jan-Mar       Impact factor: 1.577

10.  Detection of viral genomes in cultured cells and paraffin-embedded tissue sections using biotin-labeled hybridization probes.

Authors:  D J Brigati; D Myerson; J J Leary; B Spalholz; S Z Travis; C K Fong; G D Hsiung; D C Ward
Journal:  Virology       Date:  1983-04-15       Impact factor: 3.616
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