Literature DB >> 1237881

Application of fluorescamine to the study of protein-DNA interactions.

J Bode.   

Abstract

The reactivity of alpha-amino groups of basic proteins towards fluorescamine is essentially abolished if salt linkages with DNA phosphate groups are formed. This observation prompted the elaboration of a very general assay which allows the determination of binding parameters for the interaction of proteins with DNA and chromatin. Protamines, labeled with fluorescamine prior to their binding by DNA appear to be useful probes to monitor the formation and nature of DNA-protein complexes.

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Year:  1975        PMID： 1237881      PMCID： PMC343559          DOI： 10.1093/nar/2.10.1951

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  16 in total

1.  Histones F2a1 and F3 interact reversibly and cooperatively with DNA to form an equimolar complex in chromatin.

Authors:  D R Burton; J E Hyde; I O Walker
Journal:  FEBS Lett       Date:  1975-07-15       Impact factor: 4.124

2.  The use of subunit exchange chromatography for the group specific fractionation of histones.

Authors:  J Bode; K G Wagner
Journal:  Biochem Biophys Res Commun       Date:  1975-02-17       Impact factor: 3.575

3.  Modification of histone binding in calf thymus chromatin by protamine.

Authors:  T K Wong; K Marushige
Journal:  Biochemistry       Date:  1975-01-14       Impact factor: 3.162

4.  Reactive properties of the amino groups of histones in calf thymus chromatin.

Authors:  B Malchy; H Kaplan
Journal:  J Mol Biol       Date:  1974-02-05       Impact factor: 5.469

5.  Enzymatic modifications of the protamines. II. Separation and characterization of phosphorylated species of protamines from trout testis.

Authors:  A J Louie; G H Dixon
Journal:  Can J Biochem       Date:  1974-06

6.  Studies on the reaction of fluorescamine with primary amines.

Authors:  S De Bernardo; M Weigele; V Toome; K Manhart; W Leimgruber; P Böhlen; S Stein; S Udenfriend
Journal:  Arch Biochem Biophys       Date:  1974-07       Impact factor: 4.013

7.  Studies on the kinetics of reaction and hydrolysis of fluorescamine.

Authors:  S Stein; P Böhlen; S Udenfriend
Journal:  Arch Biochem Biophys       Date:  1974-07       Impact factor: 4.013

8.  Helix-coil transition and conformational studies of protamine-DNA complexes.

Authors:  S S Yu; H J Li
Journal:  Biopolymers       Date:  1973-12       Impact factor: 2.505

9.  Transformation of trout testis chromatin.

Authors:  K Marushige; G H Dixon
Journal:  J Biol Chem       Date:  1971-09-25       Impact factor: 5.157

10.  A new method for fractionation of protamines and the amino acid sequences of salmine and three components of iridine.

Authors:  T Ando; S Watanabe
Journal:  Int J Protein Res       Date:  1969
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  4 in total

1.  Cooperative binding of fluorescein-labeled clupeine by DNA.

Authors:  K Wehling; S Krauss; K G Wagner
Journal:  Nucleic Acids Res       Date:  1976-01       Impact factor: 16.971

2.  The binding of an avian myeloblastosis virus basic 12,000 dalton protein to nucleic acids.

Authors:  B J Smith; J M Bailey
Journal:  Nucleic Acids Res       Date:  1979-12-11       Impact factor: 16.971

3.  Phosphorylated protamines. I. Binding stoichiometry and thermal stability of complexes in DNA.

Authors:  L Willmitzer; J Bode; K G Wagner
Journal:  Nucleic Acids Res       Date:  1977-01       Impact factor: 16.971

4.  The binding of protamines to DNA; role of protamine phosphorylation.

Authors:  L Willmitzer; K G Wagner
Journal:  Biophys Struct Mech       Date:  1980
  4 in total

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