A Schröder1, G Delling, E A Kaiser. 1. Abteilung Osteopathologie/Zentrum für Biomechanik, Pathologisches Institut, Universitätskrankenhaus Hamburg-Eppendorf, Hamburg, Germany.
Abstract
AIMS: Expression analysis of the protein tyrosine kinases, focal adhesion kinase (FAK) and proline-rich tyrosine kinase2 (Pyk2) in high grade osteosarcomas. MATERIALS AND METHODS: Expression of the kinases was evaluated qualitatively by immunohistochemical staining and quantitatively by real-time PCR. RESULTS: Osteoblastic cells of high grade osteosarcomas show a distinct FAK expression but an overexpression at the transcriptional level could not be detected. The Pyk2-mRNA expression was decreased in osteosarcomas. CONCLUSION: An altered relationship of FAK and Pyk2 was observed for different tumors and could also be important for osteosarcoma development.
AIMS: Expression analysis of the protein tyrosine kinases, focal adhesion kinase (FAK) and proline-rich tyrosine kinase2 (Pyk2) in high grade osteosarcomas. MATERIALS AND METHODS: Expression of the kinases was evaluated qualitatively by immunohistochemical staining and quantitatively by real-time PCR. RESULTS: Osteoblastic cells of high grade osteosarcomas show a distinct FAK expression but an overexpression at the transcriptional level could not be detected. The Pyk2-mRNA expression was decreased in osteosarcomas. CONCLUSION: An altered relationship of FAK and Pyk2 was observed for different tumors and could also be important for osteosarcoma development.