Acquired cell-mediated protection in rainbow trout, Oncorhynchus mykiss, against the haemoflagellate, Cryptobia salmositica.

Disease-free rainbow trout, Oncorhynchus mykiss (Walbaum),were inoculated with either the pathogenic Cryptobia salmositica Katz, 1951 or with the attenuated vaccine strain of the parasite. A number of vaccinated fish were then challenged at 6 weeks post-vaccination with pathogenic C. salmositica. Respiratory burst activity in stimulated macrophages (isolated from infected or vaccinated fish) was demonstrated by detection of released super-oxide anions, and the protein contents in these cells were also determined after the cells were digested. At 5 weeks after infection, the respiratory burst activity of macrophages from infected fish was significantly higher than those in naive and vaccinated fish. In addition, macrophages from vaccinated fish had greater activity than naive fish. Macrophage activity in vaccinated and challenged fish was comparable to that of infected fish. Antibodies were detected (ELISA) at 3 weeks after vaccination and at 5 weeks in infected fish. Complement-fixing antibodies (immune lysis test) were detected in infected fish at 5 weeks post-infection but were not detected in vaccinated fish unless they were challenged with the pathogen, in which case the titre rose rapidly.