Literature DB >> 12374843

groEL expression in gyrB mutants of Borrelia burgdorferi.

Janet Alverson1, D Scott Samuels.   

Abstract

GroEL protein and groEL mRNA transcript were up-regulated in gyrB mutants of Borrelia burgdorferi, a causative agent of Lyme disease. Furthermore, the protein and transcript levels in gyrB mutants were greater than those in experimentally heat-shocked cultures of wild-type B. burgdorferi. Circular DNA in the gyrB mutants was more relaxed than in wild-type cells, although groEL is on the linear chromosome of B. burgdorferi. To our knowledge, this is the first evidence, albeit indirect, for the effect of DNA topology on gene expression from a linear DNA molecule in a bacterium.

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Year:  2002        PMID： 12374843      PMCID： PMC135381          DOI： 10.1128/JB.183.21.6069-6072.2002

Source DB:  PubMed          Journal:  J Bacteriol        ISSN： 0021-9193            Impact factor:   3.490


  25 in total

1.  Proteins binding to the promoter region of the operon encoding the major outer surface proteins OspA and OspB of Borrelia burgdorferi.

Authors:  N Margolis; D S Samuels
Journal:  Mol Biol Rep       Date:  1995       Impact factor: 2.316

2.  Effect of the deletion of the sigma 32-dependent promoter (P1) of the Escherichia coli topoisomerase I gene on thermotolerance.

Authors:  H Qi; R Menzel; Y C Tse-Dinh
Journal:  Mol Microbiol       Date:  1996-08       Impact factor: 3.501

3.  Increase in synthesis and stability of sigma 32 on treatment with inhibitors of DNA gyrase in Escherichia coli.

Authors:  T Mizushima; Y Ohtsuka; H Mori; T Miki; K Sekimizu
Journal:  Mol Gen Genet       Date:  1996-12-13

4.  Electrotransformation of the spirochete Borrelia burgdorferi.

Authors:  D S Samuels
Journal:  Methods Mol Biol       Date:  1995

5.  Co-induction of DNA relaxation and synthesis of DnaK and GroEL proteins in Escherichia coli by expression of LetD (CcdB) protein, an inhibitor of DNA gyrase encoded by the F factor.

Authors:  T Kaneko; T Mizushima; Y Ohtsuka; K Kurokawa; K Kataoka; T Miki; K Sekimizu
Journal:  Mol Gen Genet       Date:  1996-03-20

6.  DnaK heat shock protein of Escherichia coli maintains the negative supercoiling of DNA against thermal stress.

Authors:  Y Ogata; T Mizushima; K Kataoka; K Kita; T Miki; K Sekimizu
Journal:  J Biol Chem       Date:  1996-11-15       Impact factor: 5.157

7.  gyrB mutations in coumermycin A1-resistant Borrelia burgdorferi.

Authors:  D S Samuels; R T Marconi; W M Huang; C F Garon
Journal:  J Bacteriol       Date:  1994-05       Impact factor: 3.490

8.  Isolation of dnaJ, dnaK, and grpE homologues from Borrelia burgdorferi and complementation of Escherichia coli mutants.

Authors:  K Tilly; R Hauser; J Campbell; G J Ostheimer
Journal:  Mol Microbiol       Date:  1993-02       Impact factor: 3.501

9.  Genetic transformation of the Lyme disease agent Borrelia burgdorferi with coumarin-resistant gyrB.

Authors:  D S Samuels; K E Mach; C F Garon
Journal:  J Bacteriol       Date:  1994-10       Impact factor: 3.490

10.  Transcriptional analyses and mapping of the ospC gene in Lyme disease spirochetes.

Authors:  R T Marconi; D S Samuels; C F Garon
Journal:  J Bacteriol       Date:  1993-02       Impact factor: 3.490
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  8 in total

Review 1.  Genetic Manipulation of Borrelia Spp.

Authors:  Dan Drecktrah; D Scott Samuels
Journal:  Curr Top Microbiol Immunol       Date:  2018       Impact factor: 4.291

2.  Genetic Transformation and Complementation.

Authors:  D Scott Samuels; Dan Drecktrah; Laura S Hall
Journal:  Methods Mol Biol       Date:  2018

Review 3.  Genetics of Borrelia burgdorferi.

Authors:  Dustin Brisson; Dan Drecktrah; Christian H Eggers; D Scott Samuels
Journal:  Annu Rev Genet       Date:  2012-09-04       Impact factor: 16.830

4.  aadA confers streptomycin resistance in Borrelia burgdorferi.

Authors:  Kristi L Frank; Sharyl F Bundle; Michele E Kresge; Christian H Eggers; D Scott Samuels
Journal:  J Bacteriol       Date:  2003-11       Impact factor: 3.490

Review 5.  Changing of the guard: How the Lyme disease spirochete subverts the host immune response.

Authors:  George Chaconas; Mildred Castellanos; Theodore B Verhey
Journal:  J Biol Chem       Date:  2019-11-21       Impact factor: 5.157

Review 6.  Genetic Manipulation of Borrelia.

Authors:  Patricia A Rosa; Mollie W Jewett
Journal:  Curr Issues Mol Biol       Date:  2020-12-10       Impact factor: 2.081

7.  Fluorescent Proteins, Promoters, and Selectable Markers for Applications in the Lyme Disease Spirochete Borrelia burgdorferi.

Authors:  Constantin N Takacs; Zachary A Kloos; Molly Scott; Patricia A Rosa; Christine Jacobs-Wagner
Journal:  Appl Environ Microbiol       Date:  2018-11-30       Impact factor: 4.792

8.  A CRISPR interference platform for selective downregulation of gene expression in Borrelia burgdorferi.

Authors:  Constantin N Takacs; Molly Scott; Yunjie Chang; Zachary A Kloos; Irnov Irnov; Patricia A Rosa; Jun Liu; Christine Jacobs-Wagner
Journal:  Appl Environ Microbiol       Date:  2020-11-30       Impact factor: 4.792
  8 in total

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