Acute ethanol administration produces specific patterns of localization of Fos-immunoreactivity in the cerebellum and inferior olive of two inbred strains of mice.

Genes play an important role in behavioral responses to ethanol. We examined the response of neurons within the inferior olivary complex (IO) and cerebellum of C57Bl6/J and C3H/HeJ mice to acute ethanol, using immunodetection of Fos (Fos-IR) protein as a marker of neuronal activation. The results demonstrate specific but different patterns of Fos-IR within the IO and cerebellum, especially lobule IX, in each strain. The Fos-IR banding pattern seen in the granule cells of lobule IX is aligned with a previously described banding pattern of Purkinje cells that constitutively expressed heat-shock protein-25 (HSP-25).