BACKGROUND & AIMS: Toll-like receptors (TLRs) for bacterial constitutes are expressed not only by phagocytes but also by some subsets of T cells. We previously reported that natural killer T cells (NKT cells) play an important role in liver injury induced by Salmonella infection. In the present study, we investigated whether TLRs on NKT cells are involved in Salmonella-induced liver injury. METHODS: Gene expression of TLR2 was examined in sorted natural killer, NKT, and T cells from livers of naive mice by the reverse-transcription polymerase chain reaction method. Serum alanine aminotransferase level and FasL expression on liver lymphocytes were examined in TLR2-deficient (TLR2(-/-)) and FasL-deficient gld/gld mice before and after intraperitoneal inoculation of Salmonella choleraesuis 31N-1 using an enzyme-linked immunosorbent assay and flow cytometry. RESULTS: TLR2 gene was abundantly expressed by NKT cells freshly isolated from naive mice. FasL expression on liver NKT cells increased in TLR2(+/-) mice but not in TLR2(-/-) mice after Salmonella infection. Serum alanine aminotransferase level was significantly lower in the TLR2(-/-) and gld/gld mice than in the control mice after infection. CONCLUSIONS: TLR2 may contribute to liver injury induced by Salmonella infection via FasL induction on liver NKT cells.
BACKGROUND & AIMS: Toll-like receptors (TLRs) for bacterial constitutes are expressed not only by phagocytes but also by some subsets of T cells. We previously reported that natural killer T cells (NKT cells) play an important role in liver injury induced by Salmonella infection. In the present study, we investigated whether TLRs on NKT cells are involved in Salmonella-induced liver injury. METHODS: Gene expression of TLR2 was examined in sorted natural killer, NKT, and T cells from livers of naive mice by the reverse-transcription polymerase chain reaction method. Serum alanine aminotransferase level and FasL expression on liver lymphocytes were examined in TLR2-deficient (TLR2(-/-)) and FasL-deficient gld/gld mice before and after intraperitoneal inoculation of Salmonella choleraesuis 31N-1 using an enzyme-linked immunosorbent assay and flow cytometry. RESULTS:TLR2 gene was abundantly expressed by NKT cells freshly isolated from naive mice. FasL expression on liver NKT cells increased in TLR2(+/-) mice but not in TLR2(-/-) mice after Salmonella infection. Serum alanine aminotransferase level was significantly lower in the TLR2(-/-) and gld/gld mice than in the control mice after infection. CONCLUSIONS:TLR2 may contribute to liver injury induced by Salmonella infection via FasL induction on liver NKT cells.
Authors: A Ogawa; T Tagawa; H Nishimura; T Yajima; T Abe; T Arai; M Taniguchi; K Takeda; S Akira; Y Nimura; Y Yoshikai Journal: Gut Date: 2005-08-23 Impact factor: 23.059
Authors: Lyudmila G Burdelya; Craig M Brackett; Bojidar Kojouharov; Ilya I Gitlin; Katerina I Leonova; Anatoli S Gleiberman; Semra Aygun-Sunar; Jean Veith; Christopher Johnson; Gary J Haderski; Patricia Stanhope-Baker; Shyam Allamaneni; Joseph Skitzki; Ming Zeng; Elena Martsen; Alexander Medvedev; Dmitry Scheblyakov; Nataliya M Artemicheva; Denis Y Logunov; Alexander L Gintsburg; Boris S Naroditsky; Sergei S Makarov; Andrei V Gudkov Journal: Proc Natl Acad Sci U S A Date: 2013-04-29 Impact factor: 11.205
Authors: Mariagrazia Valentini; Alessia Piermattei; Gabriele Di Sante; Giuseppe Migliara; Giovanni Delogu; Francesco Ria Journal: J Immunol Res Date: 2014-07-24 Impact factor: 4.818