| Literature DB >> 12360232 |
Michael T McManus1, Phillip A Sharp.
Abstract
Among the 3 billion base pairs of the human genome, there are approximately 30,000-40,000 protein-coding genes, but the function of at least half of them remains unknown. A new tool - short interfering RNAs (siRNAs) - has now been developed for systematically deciphering the functions and interactions of these thousands of genes. siRNAs are an intermediate of RNA interference, the process by which double-stranded RNA silences homologous genes. Although the use of siRNAs to silence genes in vertebrate cells was only reported a year ago, the emerging literature indicates that most vertebrate genes can be studied with this technology.Entities:
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Year: 2002 PMID： 12360232 DOI： 10.1038/nrg908
Source DB: PubMed Journal: Nat Rev Genet ISSN： 1471-0056 Impact factor: 53.242