| Literature DB >> 12359222 |
Ulrich Kintscher1, Shu Wakino, Dennis Bruemmer, Stephan Goetze, Kristof Graf, Willa A Hsueh, Ronald E Law.
Abstract
The nuclear hormone receptor peroxisome proliferator-activated gamma (PPARgamma) is expressed as two isoforms (PPARgamma1 and gamma2), and is an important modulator of monocyte gene regulation and function. TGF-beta(1) is an essential and potent immune modulator and we therefore examined its effect on PPARgamma expression in human THP-1 monocytes. TGF-beta(1) strongly induced PPARgamma2 mRNA and protein expression with a lesser effect on PPARgamma1. Transcription from a PPARgamma2 promoter/luciferase reporter vector was activated approximately 3-fold by TGF-beta(1). Mutation of two C/EBP elements within the PPARgamma2 promoter reduced TGF-beta(1)-induced transcription by approximately 65%. TGF-beta(1) also induced the expression of three C/EBPisoforms (alpha, beta, and delta). Induction of PPARgamma1 and gamma2 in monocytes by TGF-beta(1) may contribute to the anti-inflammatory effects of this growth factor.Entities:
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Year: 2002 PMID： 12359222 DOI： 10.1016/s0006-291x(02)02264-7
Source DB: PubMed Journal: Biochem Biophys Res Commun ISSN： 0006-291X Impact factor: 3.575