Literature DB >> 1235911

Chinese hamster X mouse hybrid cells segregating mouse chromosomes and isozymes.

J D Minna, T H Marshall, P V Shaffer-Berman.   

Abstract

Hybrid cells are readily formed by fusing clonal Chinese hamster cells to fresh, noncultured, adult mouse spleen cells followed by isolation in selective medium. The vast majority of such hybrids retain Chinese hamster chromosomes and isozymes while segregating mouse chromosomes and isozymes. The growth, plating efficiency, ease of karyology, and rapid segregation of mouse markers allows linkage tests in primary clones. Analysis of 13 isozymes showed 12 to be asyntenic and on epair (PGD-PGM2) to be syntenic This system will allow extensive somatic cell hybrid gene mapping in the mouse and permit a comparison of human and mouse linkage relationships.

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Year:  1975        PMID： 1235911     DOI： 10.1007/bf01538667

Source DB:  PubMed          Journal:  Somatic Cell Genet        ISSN： 0098-0366


  23 in total

Review 1.  Mouse X chromosome.

Authors:  S D Brown; P Avner; G E Herman
Journal:  Mamm Genome       Date:  1992       Impact factor: 2.957

Review 2.  Mouse X chromosome.

Authors:  S D Brown; P Avner; V M Chapman; R M Hamvas; G E Herman
Journal:  Mamm Genome       Date:  1991       Impact factor: 2.957

3.  Homologous genes for enolase, phosphogluconate dehydrogenase, phosphoglucomutase, and adenylate kinase are syntenic on mouse chromosome 4 and human chromosome 1p.

Authors:  P A Lalley; U Francke; J D Minna
Journal:  Proc Natl Acad Sci U S A       Date:  1978-05       Impact factor: 11.205

4.  A somatic cell hybrid panel for mouse gene mapping characterized by PCR and FISH.

Authors:  P Williamson; S Holt; S Townsend; Y Boyd
Journal:  Mamm Genome       Date:  1995-06       Impact factor: 2.957

5.  The isolation and mapping of PCR markers specific to mouse Chromosome 2.

Authors:  S Malas; A Pilz; P A Lalley; L Pate; D A Stephenson; C Abbott
Journal:  Mamm Genome       Date:  1996-02       Impact factor: 2.957

6.  I/Lyn mouse phosphorylase kinase deficiency: mutation disrupts expression of the alpha/alpha'-subunit mRNAs.

Authors:  P K Bender; P A Lalley
Journal:  Proc Natl Acad Sci U S A       Date:  1989-12       Impact factor: 11.205

7.  Comparative mapping using somatic cell hybrids.

Authors:  J D Minna; P A Lalley; U Francke
Journal:  In Vitro       Date:  1976-11

8.  Chromosome assignments of four mouse cellular homologs of sarcoma and leukemia virus oncogenes.

Authors:  A Y Sakaguchi; P A Lalley; B U Zabel; R W Ellis; E M Scolnick; S L Naylor
Journal:  Proc Natl Acad Sci U S A       Date:  1984-01       Impact factor: 11.205

9.  Localization of cytochrome P1-450 and P3-450 genes to mouse chromosome 9.

Authors:  R H Tukey; P A Lalley; D W Nebert
Journal:  Proc Natl Acad Sci U S A       Date:  1984-05       Impact factor: 11.205

10.  Cellular homologs of the avian erythroblastosis virus erb-A and erb-B genes are syntenic in mouse but asyntenic in man.

Authors:  B U Zabel; R E Fournier; P A Lalley; S L Naylor; A Y Sakaguchi
Journal:  Proc Natl Acad Sci U S A       Date:  1984-08       Impact factor: 11.205
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