| Literature DB >> 12354924 |
Hiroyuki Fukami1, Tomiko Asakura, Hiroshi Hirano, Keiko Abe, Koichiro Shimomura, Takashi Yamakawa.
Abstract
In Atropa belladonna hairy roots, exogeneously added salicylic acid (SA) is converted to methyl salicylate (MSA) through the reaction, which might be catalysed by S-adenosyl-L-methionine: salicylic acid carboxyl methyltransferase (SAMT). Here we cloned a cDNA for A. belladonna SAMT (AbSAMT1), which consisted of 357 aa residues. It was expressed in E. coli, and the recombinant AbSAMT1 showed SAMT activity. When A. belladonna hairy roots were exposed to a high concentration of SA, AbSAMT1 mRNA begins to be expressed 12 h after the exposure, and steady expression continued over 144 h.Entities:
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Year: 2002 PMID： 12354924 DOI： 10.1093/pcp/pcf119
Source DB: PubMed Journal: Plant Cell Physiol ISSN： 0032-0781 Impact factor: 4.927