Literature DB >> 12353586

Extraction of human nuclear DNA from feces samples using the QIAamp DNA Stool Mini Kit.

Nicholas Vandenberg1, Roland A H van Oorschot.   

Abstract

The use of a QIAamp DNA Stool Mini Kit (QIAGEN) for extracting human nuclear DNA from feces samples is reported. This method employs a stool lysis buffer and a unique matrix (InhibitEX tablet) to remove PCR inhibitory substances specific to feces samples. DNA extracted from various amounts of stool and from stool samples exposed to different environmental impacts was successfully amplified and typed using the Profiler Plus Amplification Kit and ABI PRISM 310 Genetic Analyser.

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Year:  2002        PMID: 12353586

Source DB:  PubMed          Journal:  J Forensic Sci        ISSN: 0022-1198            Impact factor:   1.832


  3 in total

1.  Microbial population analysis improves the evidential value of faecal traces in forensic investigations.

Authors:  Frederike C A Quaak; Mei-Lan M de Graaf; Rob Weterings; Irene Kuiper
Journal:  Int J Legal Med       Date:  2016-05-28       Impact factor: 2.686

2.  Molecular detection of Enteropathogens from diarrheic stool of HIV positive patients in Gondar, Ethiopia.

Authors:  Lubaba Seid; William Stokes; Abebe Genetu Bayih; Sisay Getie; Aberham Abere; Habtie Tesfa; Dylan R Pillai
Journal:  BMC Infect Dis       Date:  2018-07-31       Impact factor: 3.090

3.  Phylogenetic microbiota profiling in fecal samples depends on combination of sequencing depth and choice of NGS analysis method.

Authors:  Sukithar K Rajan; Mårten Lindqvist; Robert Jan Brummer; Ida Schoultz; Dirk Repsilber
Journal:  PLoS One       Date:  2019-09-17       Impact factor: 3.240

  3 in total

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