BACKGROUND: Changes of albumin conformation flexibility and binding characteristics in plasma products can be determined by electron spin resonance spectroscopy (ESR) with spin probes as reporter molecules. METHODS: Quality of albumin transport was analyzed in different processed plasma units using a new ESR method which provides estimation of albumin properties by examination of albumin molecular structure changes. RESULTS: In serum of healthy donors could not be obtained any abnormalities. Transport function of albumin in plasma products can be influenced by various methods of plasma collection, preparation, and filtration. Evident are changes in capacity of fatty acid binding sites and ordering changes of bound substrates. Transport respiratory detoxification efficiency is decreased between 30% and 70% in all FFP compared to control values. CONCLUSIONS: ESR spectroscopy can be used as a quality test for plasma. The ESR technique has a big potential for determination of conformation changes of macromolecules and as a global tumor marker test for donor screening.
BACKGROUND: Changes of albumin conformation flexibility and binding characteristics in plasma products can be determined by electron spin resonance spectroscopy (ESR) with spin probes as reporter molecules. METHODS: Quality of albumin transport was analyzed in different processed plasma units using a new ESR method which provides estimation of albumin properties by examination of albumin molecular structure changes. RESULTS: In serum of healthy donors could not be obtained any abnormalities. Transport function of albumin in plasma products can be influenced by various methods of plasma collection, preparation, and filtration. Evident are changes in capacity of fatty acid binding sites and ordering changes of bound substrates. Transport respiratory detoxification efficiency is decreased between 30% and 70% in all FFP compared to control values. CONCLUSIONS: ESR spectroscopy can be used as a quality test for plasma. The ESR technique has a big potential for determination of conformation changes of macromolecules and as a global tumor marker test for donor screening.
Authors: Mohamed A Badawy; Basma A Yasseen; Riem M El-Messiery; Engy A Abdel-Rahman; Aya A Elkhodiry; Azza G Kamel; Hajar El-Sayed; Asmaa M Shedra; Rehab Hamdy; Mona Zidan; Diaa Al-Raawi; Mahmoud Hammad; Nahla Elsharkawy; Mohamed El Ansary; Ahmed Al-Halfawy; Alaa Elhadad; Ashraf Hatem; Sherif Abouelnaga; Laura L Dugan; Sameh Saad Ali Journal: Elife Date: 2021-11-25 Impact factor: 8.140