Phospholipid bicelles that align with their normals parallel to the magnetic field.

We have recently reported phospholipid bicelles (bilayered micelles) that have positive anisotropy of the magnetic susceptibility and align with their normals parallel to an external magnetic field [J. Am. Chem. Soc. 2001, 123, 1537]. Improvements have been made via the synthesis of a new phospholipid, 1-dodecanoyl-2-(4-(4-biphenyl)butanoyl)-sn-glycero-3-phosphocholine (DBBPC). Bicelles can be formed by mixing DBBPC with a short-chain phospholipid, 1,2-dihexanoyl-sn-glycero-3-phosphocholine (DHPC) in a ratio between 5.1:1 and 6.5:1 in an aqueous medium. The (31)P NMR spectra clearly show that these bicelles align with their principal axes parallel to the magnetic field within a wide temperature range. The (31)P chemical shifts indicate that the conformation of the polar headgroup in these bicelles may be different from that in common bicelles. The phase behavior of a mixture of DBBPC/DHPC with 6:1 mole ratio was investigated in the temperature range of 10-75 degrees C using (31)P, (2)H, and (23)Na NMR. At lower temperatures (10-54 degrees C), the system is dominated by the bicellar phase. At higher temperatures (54-75 degrees C), isotropic micelles are formed and coexist with the bicelles. The partial alignment of maltotriose in the DBBPC/DHPC system was studied at three temperatures, and the (1)H-(13)C dipolar coupling constants are compared with those obtained for two other bicelle solutions.