Literature DB >> 12270205

Quantification of isoflavones in red clover by high-performance liquid chromatography.

Liselotte Krenn1, Iris Unterrieder, Renate Ruprechter.   

Abstract

An RP-HPLC method for the determination of daidzein, genistein, formononetin and biochanin A in red clover (Trifolium pratense L.) was developed and validated. The compounds are quantified after hydrolytic extraction using an internal standard. On a base-deactivated C(18) column good separation of the analytes, also from accompanying substances, and excellent peak shape are achieved by gradient elution with aqueous sulfuric acid and acetonitrile. The method was applied to the analysis of different red clover cultivars.

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Year:  2002        PMID： 12270205     DOI： 10.1016/s1570-0232(02)00079-x

Source DB:  PubMed          Journal:  J Chromatogr B Analyt Technol Biomed Life Sci        ISSN： 1570-0232            Impact factor:   3.205


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