OBJECTIVE: S100A6 and S100A4, two of S100 protein family, have been suggested to be associated with cancer tumorigenesis and metastasis. The aim of this study was to evaluate the expression levels of S100A6 and S100A4 in matched samples of primary human colorectal adenocarcinomas (T), adjacent normal colorectal mucosa (N) and liver metastases (M). This gave us the advantage of directly comparing levels of S100A6 and S100A4 expression within the same genetic background. METHODS: In matched samples of N, T and M from 10 colorectal adenocarcinoma patients, expressions of S100A6 and S100A4 were studied by Western blot and immunohistochemical analyses using specific antibodies against each protein. RESULTS: The expression levels of S100A6 were significantly higher in T than in N (p < 0.05), while those of S100A4 showed no difference between T and N. There were no significant differences in the expression levels of S100A6 or S100A4 between M and T. Similar results were obtained by immunohistochemical analysis. Moreover, S100A6 was stained more intensely in invading fronts than in central portions of both T and M. CONCLUSIONS: The observed differential expression of S100A6 and S100A4 suggests that S100A6, rather than S100A4, is associated with human colorectal adenocarcinoma tumorigenesis and invasion/metastasis. Copyright 2002 S. Karger AG, Basel
OBJECTIVE:S100A6 and S100A4, two of S100 protein family, have been suggested to be associated with cancer tumorigenesis and metastasis. The aim of this study was to evaluate the expression levels of S100A6 and S100A4 in matched samples of primary humancolorectal adenocarcinomas (T), adjacent normal colorectal mucosa (N) and liver metastases (M). This gave us the advantage of directly comparing levels of S100A6 and S100A4 expression within the same genetic background. METHODS: In matched samples of N, T and M from 10 colorectal adenocarcinomapatients, expressions of S100A6 and S100A4 were studied by Western blot and immunohistochemical analyses using specific antibodies against each protein. RESULTS: The expression levels of S100A6 were significantly higher in T than in N (p < 0.05), while those of S100A4 showed no difference between T and N. There were no significant differences in the expression levels of S100A6 or S100A4 between M and T. Similar results were obtained by immunohistochemical analysis. Moreover, S100A6 was stained more intensely in invading fronts than in central portions of both T and M. CONCLUSIONS: The observed differential expression of S100A6 and S100A4 suggests that S100A6, rather than S100A4, is associated with humancolorectal adenocarcinoma tumorigenesis and invasion/metastasis. Copyright 2002 S. Karger AG, Basel
Authors: Benjamin Balluff; Sandra Rauser; Stephan Meding; Mareike Elsner; Cedrik Schöne; Annette Feuchtinger; Christoph Schuhmacher; Alexander Novotny; Uta Jütting; Giuseppina Maccarrone; Hakan Sarioglu; Marius Ueffing; Herbert Braselmann; Horst Zitzelsberger; Roland M Schmid; Heinz Höfler; Matthias P Ebert; Axel Walch Journal: Am J Pathol Date: 2011-10-18 Impact factor: 4.307
Authors: Molly A Harris; Hyuna Yang; Benjamin E Low; Joydeep Mukherjee; Joydeep Mukherje; Abhijit Guha; Roderick T Bronson; Leonard D Shultz; Mark A Israel; Kyuson Yun Journal: Cancer Res Date: 2008-12-15 Impact factor: 12.701
Authors: Israel T Silva; Ricardo Z N Vêncio; Thiago Y K Oliveira; Greice A Molfetta; Wilson A Silva Journal: BMC Bioinformatics Date: 2010-03-30 Impact factor: 3.169
Authors: Christian Melle; Günther Ernst; Bettina Schimmel; Annett Bleul; Ferdinand von Eggeling Journal: PLoS One Date: 2008-12-02 Impact factor: 3.240
Authors: Christine M Costello; Nancy Mah; Robert Häsler; Philip Rosenstiel; Georg H Waetzig; Andreas Hahn; Tim Lu; Yesim Gurbuz; Susanna Nikolaus; Mario Albrecht; Jochen Hampe; Ralph Lucius; Günther Klöppel; Holger Eickhoff; Hans Lehrach; Thomas Lengauer; Stefan Schreiber Journal: PLoS Med Date: 2005-08-23 Impact factor: 11.069