Literature DB >> 12234470

Measurement of adenylyl cyclase by separating cyclic AMP on silica gel thin-layer chromatography.

Haruhiro Higashida1, Kazi Z Hossain, Hiroko Takahagi, Mami Noda.   

Abstract

A procedure for isolation of cyclic AMP (cAMP) by thin-layer chromatography on silica gel is described. One-dimensional ascending chromatograms were developed using [H(2)O/C(2)H(5)OH/NH(4)HCO(3) (30%:70%:0.2M)] as the mobile phase. This procedure separated [32P]cAMP from other radioactive metabolites of [32P]ATP in up to 19 samples on one sheet (20 x 10 cm) over 40-60 min at room temperature (21 degrees C). This simple and rapid isolation method provides a novel and convenient technique for the assay of adenylyl cyclase.

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Year:  2002        PMID： 12234470     DOI： 10.1016/s0003-2697(02)00237-3

Source DB:  PubMed          Journal:  Anal Biochem        ISSN： 0003-2697            Impact factor:   3.365


  3 in total

1.  Cyclic ADP-ribose as an endogenous inhibitor of the mTOR pathway downstream of dopamine receptors in the mouse striatum.

Authors:  Haruhiro Higashida; Shin-Ya Kamimura; Takeshi Inoue; Osamu Hori; Mohammad Saharul Islam; Olga Lopatina; Chiharu Tsuji
Journal:  J Neural Transm (Vienna)       Date:  2016-12-26       Impact factor: 3.575

2.  Cyclic AMP intoxication of macrophages by a Mycobacterium tuberculosis adenylate cyclase.

Authors:  Nisheeth Agarwal; Gyanu Lamichhane; Radhika Gupta; Scott Nolan; William R Bishai
Journal:  Nature       Date:  2009-06-10       Impact factor: 49.962

3.  Bacterial cyclic AMP-phosphodiesterase activity coordinates biofilm formation.

Authors:  Eric J Kalivoda; Kimberly M Brothers; Nicholas A Stella; Matthew J Schmitt; Robert M Q Shanks
Journal:  PLoS One       Date:  2013-07-29       Impact factor: 3.240
  3 in total

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