Literature DB >> 12233747

Isolation, purification and preliminary characterization of cryophilic proteases of marine origin.

M H Salamanca1, C Barría, J A Asenjo, B A Andrews.   

Abstract

The isolation and preliminary characterization of a trypsin-like protease with high activity at 20 degrees C is described. This protease was isolated from Antarctic krill (Euphasia superba) by a two-step chromatography process and the use of zymogram analyses. The protease has a molecular weight of 30 kDa and a pI of 4.1. Its specific activity at 20 degrees C on BAPNA is 0.5 U/mg.

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Year:  2001        PMID： 12233747     DOI： 10.1023/a:1016383212244

Source DB:  PubMed          Journal:  Bioseparation        ISSN： 0923-179X


  2 in total

1.  An alkaline protease from Bacillus circulans BM15, newly isolated from a mangrove station: characterization and application in laundry detergent formulations.

Authors:  Meera Venugopal; A V Saramma
Journal:  Indian J Microbiol       Date:  2008-01-11       Impact factor: 2.461

2.  Cold adaptation, ca2+ dependency and autolytic stability are related features in a highly active cold-adapted trypsin resistant to autoproteolysis engineered for biotechnological applications.

Authors:  Alvaro Olivera-Nappa; Fernando Reyes; Barbara A Andrews; Juan A Asenjo
Journal:  PLoS One       Date:  2013-08-12       Impact factor: 3.240
  2 in total

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