Characterization of the Insulin and Transferrin Receptors of Two Cancer Cell Lines.

The insulin and transferrin receptors of the PA-1 and GR2H6 cell lines were characterized respectively. The optimum pH for the binding of PA-1 cell is 7.0 with insulin and with transferrin is 7.5 8.0. Scatchard plot and Hill plot analysis show respectively the receptor number of 2.97x10(6)/cell and 6.21x10(7)/cell for insulin receptors of PA-1 and GR2H6 with kD of 4.16x10(-7) M and 7.57x10(-7) M, Hill coefficient of 0.78 and 0.82, showing a negative cooperativity; transferring receptors of PA-1 and GR2H6 were found to have respectively receptor number of 1.25x10(5)/cell and 1.40x10(5)/cell, with kD of 1.38x10(-9) M and 1.66x10(-8) M. The Hill coefficient was 1.04 and 0.98, almost no different from 1, so transferrin receptors showed no cooperativity.