Literature DB >> 12230515

How fast are UV-dimers repaired in human skin DNA in situ?

Kari Hemminki1, Erna Snellman.   

Abstract

Sheehan et al (2002) published a paper on UV-dimers in human skin and their repair with contradictions in their own data. In one experiment they applied two doses, 0.65 and 2 MED, but the apparent dimer levels, measured by a immunohistochemical technique, only increased in skin of type II (Fig 2a). The authors offered as explanation that the level of DNA damage would saturate. The dimer levels reached are at around 1 dimer per 1 million nucleotides, and there has been no evidence for saturation when a quantitative postlabeling technique has been used (Bykov et al, 1998). There is no reason for saturation until most of the thydmidyl-thymidine positions would be occupied in DNA, which, based on literature with other intrastrand cross-linkers, would require dimer levels 1 : 100 (Försti et al, 1989), or 10,000 times higher levels of dimer than that obtained by an irradiation at a MED dose.

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Year:  2002        PMID: 12230515     DOI: 10.1046/j.1523-1747.2002.00289.x

Source DB:  PubMed          Journal:  J Invest Dermatol        ISSN: 0022-202X            Impact factor:   8.551


  2 in total

Review 1.  Changes in DNA repair during aging.

Authors:  Vera Gorbunova; Andrei Seluanov; Zhiyong Mao; Christpher Hine
Journal:  Nucleic Acids Res       Date:  2007-10-02       Impact factor: 16.971

2.  Concomitant DNA methylation and transcriptome signatures define epidermal responses to acute solar UV radiation.

Authors:  Nicholas Holzscheck; Jörn Söhle; Torsten Schläger; Cassandra Falckenhayn; Elke Grönniger; Ludger Kolbe; Horst Wenck; Lara Terstegen; Lars Kaderali; Marc Winnefeld; Katharina Gorges
Journal:  Sci Rep       Date:  2020-07-31       Impact factor: 4.379

  2 in total

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