Literature DB >> 12230489

The smooth fractionator.

H J G Gundersen1.   

Abstract

A modification of the general fractionator sampling technique called the smooth fractionator is presented. It may be used in almost every situation in which sampling is performed from distinct items that are uniquely defined, often they are physically separated items or clusters like pieces, blocks, slabs, sections, etc. To each item is associated a 'guesstimate' or an associated variable with a more-or-less close--and possibly biased--relationship to the content of the item. The smooth fractionator is systematic sampling among the items arranged according to the guesstimates in a unique, symmetric sequence with one peak and minimal jumps. The smooth fractionator is both very simple to implement and so efficient that it should probably always be used unless the natural sequence of the sampling items is equally smooth. So far, there is no theory for the prediction of the efficiency of smooth fractionator designs in general, and their properties are therefore illustrated with a range of real and simulated examples. At the cost of a slightly more elaborate sampling scheme, it is, however, always possible to obtain an unbiased estimate of the real precision and of some of the variance components. The only real practical problem for always obtaining a high precision with the smooth fractionator is specimen inhomogeneity, but that is detectable at almost no extra cost. With careful designs and for sample sizes of about 10, the sampling variation for the primary, smooth fractionator sampling step may in practice often be small enough to be ignored.

Mesh:

Year:  2002        PMID: 12230489     DOI: 10.1046/j.1365-2818.2002.01054.x

Source DB:  PubMed          Journal:  J Microsc        ISSN: 0022-2720            Impact factor:   1.758


  51 in total

Review 1.  The use of design-based stereology to evaluate volumes and numbers in the liver: a review with practical guidelines.

Authors:  Ricardo Marcos; Rogério A F Monteiro; Eduardo Rocha
Journal:  J Anat       Date:  2012-02-01       Impact factor: 2.610

2.  Reduced glutamate decarboxylase 65 protein within primary auditory cortex inhibitory boutons in schizophrenia.

Authors:  Caitlin E Moyer; Kristen M Delevich; Kenneth N Fish; Josephine K Asafu-Adjei; Allan R Sampson; Karl-Anton Dorph-Petersen; David A Lewis; Robert A Sweet
Journal:  Biol Psychiatry       Date:  2012-05-23       Impact factor: 13.382

3.  Rapid antidepressant effect of ketamine correlates with astroglial plasticity in the hippocampus.

Authors:  Maryam Ardalan; Ali H Rafati; Jens R Nyengaard; Gregers Wegener
Journal:  Br J Pharmacol       Date:  2017-02-08       Impact factor: 8.739

Review 4.  Efficient quantitative morphological phenotyping of genetically altered organisms using stereology.

Authors:  John Milton Lucocq
Journal:  Transgenic Res       Date:  2006-11-14       Impact factor: 2.788

5.  Effect of severe calorie restriction on the lung in two strains of mice.

Authors:  John M Bishai; Wayne Mitzner
Journal:  Am J Physiol Lung Cell Mol Physiol       Date:  2008-05-30       Impact factor: 5.464

6.  An official research policy statement of the American Thoracic Society/European Respiratory Society: standards for quantitative assessment of lung structure.

Authors:  Connie C W Hsia; Dallas M Hyde; Matthias Ochs; Ewald R Weibel
Journal:  Am J Respir Crit Care Med       Date:  2010-02-15       Impact factor: 21.405

7.  From gross anatomy to the nanomorphome: stereological tools provide a paradigm for advancing research in quantitative morphomics.

Authors:  Terry M Mayhew; John M Lucocq
Journal:  J Anat       Date:  2015-03-09       Impact factor: 2.610

8.  No change in neuron numbers in the dentate nucleus of patients with schizophrenia estimated with a new stereological method--the smooth fractionator.

Authors:  B B Andersen; K Fabricius; H J G Gundersen; J Jelsing; A K Stark
Journal:  J Anat       Date:  2004-10       Impact factor: 2.610

9.  Treatment with a proton pump inhibitor improves glycaemic control in Psammomys obesus, a model of type 2 diabetes.

Authors:  T B Bödvarsdóttir; K D Hove; C F Gotfredsen; L Pridal; A Vaag; A E Karlsen; J S Petersen
Journal:  Diabetologia       Date:  2010-06-30       Impact factor: 10.122

10.  Inhibition of the nuclear factor-κB pathway prevents beta cell failure and diet induced diabetes in Psammomys obesus.

Authors:  Josefine Friberg; Morten F Tonnesen; Schott Heller; Flemming Pociot; Thóra B Bödvarsdottir; Allan E Karlsen
Journal:  PLoS One       Date:  2010-10-11       Impact factor: 3.240

View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.