Determination of the content of desmopressin in pharmaceutical preparations by HPLC and validation of the method.

The aim of this study was to apply high performance liquid chromatography to the determination of content of desmopressin in pharmaceutical preparations and validation of the method. The satisfactory results have been obtained using a column Luna C 8.5 microm, 100 x 4.6 mm and a mobile phase containing 0.067 M phosphate buffer of pH = 7 and acetonitrile in the proportion 83:17. It has been shown that the elaborated method shows good precision and accuracy and can be applied to the qualitative and quantitative analysis of pharmaceutical preparations containing desmopressin.