Literature DB >> 12228455

Subcellular Compartmentation of the 4-Aminobutyrate Shunt in Protoplasts from Developing Soybean Cotyledons.

K. E. Breitkreuz1, B. J. Shelp.   

Abstract

The subcellular localization of enzymes involved in the 4-ami-nobutyrate shunt was investigated in protoplasts prepared from developing soybean [Glycine max (L.) Merrill cv Maple Arrow] cotyledons. Protoplast lysate was fractionated by differential and continuous Percoll-gradient centrifugation to separate organelle fractions. Glutamate decarboxylase (EC 4.1.1.15) was found exclusively in the cytosol, whereas 4-aminobutyrate:pyruvate transami-nase (EC 2.6.1.19) and succinic semialdehyde dehydrogenase (EC 1.2.1.16) were associated exclusively with the mitochondrial fractions. Mitochondrial fractions also catabolized [U-14C]4-aminobu-tyrate to labeled succinate.

Entities:  

Year:  1995        PMID： 12228455      PMCID： PMC157309          DOI： 10.1104/pp.108.1.99

Source DB:  PubMed          Journal:  Plant Physiol        ISSN： 0032-0889            Impact factor:   8.340


  13 in total

1.  Catalase in vitro.

Authors:  H Aebi
Journal:  Methods Enzymol       Date:  1984       Impact factor: 1.600

2.  The reaction of amino-oxyacetate with pyridoxal phosphate-dependent enzymes.

Authors:  R A John; A Charteris
Journal:  Biochem J       Date:  1978-06-01       Impact factor: 3.857

3.  Purification and characterization of an anaerobically induced alanine aminotransferase from barley roots.

Authors:  A G Good; D G Muench
Journal:  Plant Physiol       Date:  1992-08       Impact factor: 8.340

4.  Method to Obtain a Chlorophyll-free Preparation of Intact Mitochondria from Spinach Leaves.

Authors:  A Bergman; P Gardeström; I Ericson
Journal:  Plant Physiol       Date:  1980-09       Impact factor: 8.340

5.  Proton/l-Glutamate Symport and the Regulation of Intracellular pH in Isolated Mesophyll Cells.

Authors:  W A Snedden; I Chung; R H Pauls; A W Bown
Journal:  Plant Physiol       Date:  1992-06       Impact factor: 8.340

6.  Alcohol Dehydrogenase and Pyruvate Decarboxylase Activity in Leaves and Roots of Eastern Cottonwood (Populus deltoides Bartr.) and Soybean (Glycine max L.).

Authors:  T W Kimmerer
Journal:  Plant Physiol       Date:  1987-08       Impact factor: 8.340

7.  Rapid Accumulation of gamma-Aminobutyric Acid and Alanine in Soybean Leaves in Response to an Abrupt Transfer to Lower Temperature, Darkness, or Mechanical Manipulation.

Authors:  W Wallace; J Secor; L E Schrader
Journal:  Plant Physiol       Date:  1984-05       Impact factor: 8.340

8.  In Vivo and In Vitro Studies on gamma-Aminobutyric Acid Metabolism with the Radish Plant (Raphanus sativus, L.).

Authors:  J G Streeter; J F Thompson
Journal:  Plant Physiol       Date:  1972-04       Impact factor: 8.340

9.  Development of Microbodies in Sunflower Cotyledons and Castor Bean Endosperm during Germination.

Authors:  C Schnarrenberger; A Oeser; N E Tolbert
Journal:  Plant Physiol       Date:  1971-11       Impact factor: 8.340

10.  A plant glutamate decarboxylase containing a calmodulin binding domain. Cloning, sequence, and functional analysis.

Authors:  G Baum; Y Chen; T Arazi; H Takatsuji; H Fromm
Journal:  J Biol Chem       Date:  1993-09-15       Impact factor: 5.157
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  19 in total

1.  The Metabolism and Functions of [gamma]-Aminobutyric Acid.

Authors:  A. W. Bown; B. J. Shelp
Journal:  Plant Physiol       Date:  1997-09       Impact factor: 8.340

2.  Posttranscriptional gene silencing in nuclei.

Authors:  Paul Hoffer; Sergey Ivashuta; Olga Pontes; Alexa Vitins; Craig Pikaard; Andrew Mroczka; Nicholas Wagner; Toni Voelker
Journal:  Proc Natl Acad Sci U S A       Date:  2010-12-20       Impact factor: 11.205

3.  Subcellular compartmentation of 4-aminobutyrate (GABA) metabolism in arabidopsis: An update.

Authors:  Barry J Shelp; Adel Zarei
Journal:  Plant Signal Behav       Date:  2017-04-27

4.  Molecular cloning and differential expression of an gamma-aminobutyrate transaminase gene, OsGABA-T, in rice (Oryza sativa) leaves infected with blast fungus.

Authors:  Chunxia Wu; Shanyue Zhou; Quan Zhang; Wensheng Zhao; Youliang Peng
Journal:  J Plant Res       Date:  2006-08-08       Impact factor: 2.629

5.  Salt stress-induced proline transporters and salt stress-repressed broad specificity amino acid permeases identified by suppression of a yeast amino acid permease-targeting mutant.

Authors:  D Rentsch; B Hirner; E Schmelzer; W B Frommer
Journal:  Plant Cell       Date:  1996-08       Impact factor: 11.277

6.  Rapid [gamma]-Aminobutyric Acid Synthesis and the Inhibition of the Growth and Development of Oblique-Banded Leaf-Roller Larvae.

Authors:  A. I. Ramputh; A. W. Bown
Journal:  Plant Physiol       Date:  1996-08       Impact factor: 8.340

7.  Characterization of two glutamate decarboxylase cDNA clones from Arabidopsis.

Authors:  F J Turano; T K Fang
Journal:  Plant Physiol       Date:  1998-08       Impact factor: 8.340

8.  Biochemical characterization, mitochondrial localization, expression, and potential functions for an Arabidopsis gamma-aminobutyrate transaminase that utilizes both pyruvate and glyoxylate.

Authors:  Shawn M Clark; Rosa Di Leo; Preetinder K Dhanoa; Owen R Van Cauwenberghe; Robert T Mullen; Barry J Shelp
Journal:  J Exp Bot       Date:  2009-03-05       Impact factor: 6.992

9.  Quantification of compartmented metabolic fluxes in developing soybean embryos by employing biosynthetically directed fractional (13)C labeling, two-dimensional [(13)C, (1)H] nuclear magnetic resonance, and comprehensive isotopomer balancing.

Authors:  Ganesh Sriram; D Bruce Fulton; Vidya V Iyer; Joan Marie Peterson; Ruilian Zhou; Mark E Westgate; Martin H Spalding; Jacqueline V Shanks
Journal:  Plant Physiol       Date:  2004-10-01       Impact factor: 8.340

10.  Subcellular localization and expression of multiple tomato gamma-aminobutyrate transaminases that utilize both pyruvate and glyoxylate.

Authors:  Shawn M Clark; Rosa Di Leo; Owen R Van Cauwenberghe; Robert T Mullen; Barry J Shelp
Journal:  J Exp Bot       Date:  2009-05-21       Impact factor: 6.992
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