| Literature DB >> 12226817 |
Omana V Nainan1, Marina L Khristova, KwanSoo Byun, Guoliang Xia, Patricia E Taylor, Cladd E Stevens, Harold S Margolis.
Abstract
Variants in the amino acid composition of the primary antibody-binding site of hepatitis B surface antigen (HBsAg) have been identified in a number of populations with chronic hepatitis B virus (HBV) infection. Direct sequencing of amplified or cloned PCR products, solid phase detection of sequence-specific PCR products (SP-PCR), and limiting dilution cloning PCR (LDC-PCR) were compared to determine their sensitivity in detecting differing concentrations of HBsAg variants. LDC-PCR had the greatest sensitivity and could detect HBsAg variants at a concentration of 0.1% of the total viral population. HBsAg variants were detected in 51% of infants with chronic HBV infection acquired after postexposure prophylaxis, and more than half of the variants were detected only by the most sensitive methods. Copyright 2002 Wiley-Liss, Inc.Entities:
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Year: 2002 PMID： 12226817 DOI： 10.1002/jmv.10206
Source DB: PubMed Journal: J Med Virol ISSN： 0146-6615 Impact factor: 2.327