Literature DB >> 12226697

Evaluation of an immunochromatographic dip-strip test for the detection of Cryptosporidium oocysts in stool specimens.

M T Llorente1, A Clavel, M Varea, S Olivera, F J Castillo, J Sahagún, M C Rubio, R Gómez-Lus.   

Abstract

The study presented here examined the efficacy of a commercially available qualitative immunochromatographic assay for detecting Cryptosporidium oocysts in stool samples. A total of 75 samples were tested, including 50 positive for Cryptosporidium spp. by acid-fast stain, 20 positive for other parasites ( Blastocystis hominis, Endolimax nana, Entamoeba coli, Giardia lamblia, Ascaris lumbricoides, Strongyloides stercoralis and Trichuris trichiura), and five negative samples. The observed sensitivity was 98%, while specificity was 100%; the detection threshold was near 1000 oocysts/ml. Correctly diagnosed positive samples included Cryptosporidium parvum genotypes 1 and 2, whereas the single false-negative sample corresponded to a Cryptosporidium meleagridis infection.

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Year:  2002        PMID: 12226697     DOI: 10.1007/s10096-002-0778-1

Source DB:  PubMed          Journal:  Eur J Clin Microbiol Infect Dis        ISSN: 0934-9723            Impact factor:   3.267


  9 in total

1.  Evaluation of four commercial rapid immunochromatographic assays for detection of Cryptosporidium antigens in stool samples: a blind multicenter trial.

Authors:  Patrice Agnamey; Claudine Sarfati; Claudine Pinel; Meja Rabodoniriina; Nathalie Kapel; Emmanuel Dutoit; Cécile Garnaud; Momar Diouf; Jean-François Garin; Anne Totet; F Derouin
Journal:  J Clin Microbiol       Date:  2011-02-02       Impact factor: 5.948

2.  ImmunoCard STAT! cartridge antigen detection assay compared to microplate enzyme immunoassay and modified Kinyoun's acid-fast staining technique for detection of Cryptosporidium in fecal specimens.

Authors:  Amal Abdul-Rasheed El-Moamly; Mohamed Aly El-Sweify
Journal:  Parasitol Res       Date:  2011-08-14       Impact factor: 2.289

3.  Evaluation of an immunochromatographic dip strip test for simultaneous detection of Cryptosporidium spp, Giardia duodenalis, and Entamoeba histolytica antigens in human faecal samples.

Authors:  P Goñi; B Martín; M Villacampa; A García; C Seral; F J Castillo; A Clavel
Journal:  Eur J Clin Microbiol Infect Dis       Date:  2012-01-20       Impact factor: 3.267

4.  Low sensitivity of the ImmunocardSTAT® Crypto/Giardia Rapid Assay test for the detection of Giardia and Cryptosporidium in fecal samples from children living in Libreville, Central Africa.

Authors:  M K Bouyou-Akotet; M Owono-Medang; M N Moussavou-Boussougou; M Mabika Mamfoumbi; R Mintsa-Nguema; D P Mawili-Mboumba; M Kombila
Journal:  J Parasit Dis       Date:  2015-02-14

5.  Evaluation of ImmunoCard STAT test and ELISA versus light microscopy in diagnosis of giardiasis and cryptosporidiosis.

Authors:  H A Sadaka; M R Gaafar; R F Mady; N N Hezema
Journal:  Parasitol Res       Date:  2015-05-01       Impact factor: 2.289

6.  Cryptosporidium felis infection, Spain.

Authors:  María Teresa Llorente; Antonio Clavel; Marzo Varea; María Pilar Goñi; Juan Sahagún; Susana Olivera
Journal:  Emerg Infect Dis       Date:  2006-09       Impact factor: 6.883

7.  Performance evaluation of point-of-care test for detection of Cryptosporidium stool antigen in children and HIV infected adults.

Authors:  Techalew Shimelis; Endale Tadesse
Journal:  Parasit Vectors       Date:  2014-05-16       Impact factor: 3.876

Review 8.  Human Ascariasis: Diagnostics Update.

Authors:  Poppy H L Lamberton; Peter M Jourdan
Journal:  Curr Trop Med Rep       Date:  2015-10-03

9.  Evaluation of a Rapid Lateral Flow Point-of-Care Test for Detection of Cryptosporidium.

Authors:  Molly E Fleece; Jack Heptinstall; Shaila S Khan; Mamum Kabir; Joel Herbein; Rashidul Haque; William A Petri
Journal:  Am J Trop Med Hyg       Date:  2016-08-29       Impact factor: 2.345

  9 in total

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