Methodology for urinary 8-hydroxy-2'-deoxyguanosine analysis by HPLC with electrochemical detection.

The compound 8-hydroxy-2'-deoxyguanosine (8-OHdG), one of the major oxidative DNA base products, is a commonly used biomarker of oxidative DNA damage. The routine measurement of 8-OHdG in biologic samples, and especially in urine, is inherently a challenging analytical problem due to the low levels of the analyte and the complexity of the sample. In this study, a simple and rapid procedure based on one step solid phase extraction (SPE) followed by high-performance liquid chromatography with electrochemical detection (HPLC-ECD) analysis is described. The methodology proposed resulted in a recovery of 74.5+/-12% for urinary 8-OHdG with a detection limit of 5.0 microg l(-1).