Literature DB >> 12220668

Identification of a pH regulated Na(+)/H(+) antiporter of Methanococcus jannaschii.

Jens Hellmer1, Ralf Pätzold, Carsten Zeilinger.   

Abstract

The genome of the hyperthermophilic archaeon Methanococcus jannaschii contains three Na(+)/H(+) antiporter related genes Mj0057, Mj1521 and Mj1275. Comparative sequence alignments revealed that Mj0057 and Mj1521 belong to the NhaP family whereas Mj1275 is a member of the NapA family. The genes were cloned and expressed in the double mutant Escherichia coli strain Frag144 (DeltanhaA, DeltanhaB) to analyze their capability of mediating DeltapH driven Na(+) flux in everted vesicles. From the tested clones only Mj0057 displayed Na(+) (Li(+))/H(+) antiporter activity. The transport was pH dependent and occurred at pH 7.0 and below. At pH 6.0 the apparent K(m) values for Na(+) and Li(+) were approximately 10 and 2.5 mM, respectively.

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Year:  2002        PMID： 12220668     DOI： 10.1016/s0014-5793(02)03244-1

Source DB:  PubMed          Journal:  FEBS Lett        ISSN： 0014-5793            Impact factor:   4.124


  10 in total

1.  Structure of the archaeal Na+/H+ antiporter NhaP1 and functional role of transmembrane helix 1.

Authors:  Panchali Goswami; Cristina Paulino; Dilem Hizlan; Janet Vonck; Ozkan Yildiz; Werner Kühlbrandt
Journal:  EMBO J       Date:  2010-12-10       Impact factor: 11.598

2.  Physiology of the Vc-NhaP paralogous group of cation-proton antiporters in Vibrio cholerae.

Authors:  Muntahi Mourin; Carla B Schubiger; Craig T Resch; Claudia C Häse; Pavel Dibrov
Journal:  Mol Cell Biochem       Date:  2017-01-13       Impact factor: 3.396

3.  A proposal to rename the hyperthermophile Pyrococcus woesei as Pyrococcus furiosus subsp. woesei.

Authors:  Wirojne Kanoksilapatham; Juan M González; Dennis L Maeder; Jocelyne DiRuggiero; Frank T Robb
Journal:  Archaea       Date:  2004-10       Impact factor: 3.273

4.  pH-induced structural change in a sodium/proton antiporter from Methanococcus jannaschii.

Authors:  Kutti R Vinothkumar; Sander H J Smits; Werner Kühlbrandt
Journal:  EMBO J       Date:  2005-07-14       Impact factor: 11.598

5.  The genome sequence of Methanosphaera stadtmanae reveals why this human intestinal archaeon is restricted to methanol and H2 for methane formation and ATP synthesis.

Authors:  Wolfgang F Fricke; Henning Seedorf; Anke Henne; Markus Krüer; Heiko Liesegang; Reiner Hedderich; Gerhard Gottschalk; Rudolf K Thauer
Journal:  J Bacteriol       Date:  2006-01       Impact factor: 3.490

6.  Three putative cation/proton antiporters from the soda lake alkaliphile Alkalimonas amylolytica N10 complement an alkali-sensitive Escherichia coli mutant.

Authors:  Yi Wei; Jun Liu; Yanhe Ma; Terry A Krulwich
Journal:  Microbiology       Date:  2007-07       Impact factor: 2.777

7.  Conserved and diversified gene families of monovalent cation/h(+) antiporters from algae to flowering plants.

Authors:  Salil Chanroj; Guoying Wang; Kees Venema; Muren Warren Zhang; Charles F Delwiche; Heven Sze
Journal:  Front Plant Sci       Date:  2012-02-14       Impact factor: 5.753

8.  A bioenergetic basis for membrane divergence in archaea and bacteria.

Authors:  Víctor Sojo; Andrew Pomiankowski; Nick Lane
Journal:  PLoS Biol       Date:  2014-08-12       Impact factor: 8.029

9.  pH- and sodium-induced changes in a sodium/proton antiporter.

Authors:  Cristina Paulino; Werner Kühlbrandt
Journal:  Elife       Date:  2014-01-28       Impact factor: 8.140

10.  Keeping it simple, transport mechanism and pH regulation in Na+/H+ exchangers.

Authors:  Octavian Călinescu; Cristina Paulino; Werner Kühlbrandt; Klaus Fendler
Journal:  J Biol Chem       Date:  2014-03-18       Impact factor: 5.157
  10 in total

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