Literature DB >> 12219843

Combinatorial labelling of DNA probes enables multicolour fluorescence in situ hybridisation in plants.

Robert Hasterok1, Tim Langdon, Steve Taylor, Glyn Jenkins.   

Abstract

This paper demonstrates a simple but effective use of combinatorial probes to label plant chromosomes by multicolour fluorescence in situ hybridisation (FISH). Three different DNA probes were labelled with only two different fluorophores, hybridised to somatic metaphase chromosomes of Secale cereale and Triticum aestivum, simultaneously visualised, and unequivocally distinguished in a single FISH experiment. Combinatorial labelling can augment karyotypical investigations, physical mapping of chromosomes and other analyses in plants based upon FISH.

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Year:  2002        PMID： 12219843

Source DB:  PubMed          Journal:  Folia Histochem Cytobiol        ISSN： 0239-8508            Impact factor:   1.698


  15 in total

1.  Laying the cytotaxonomic foundations of a new model grass, Brachypodium distachyon (L.) Beauv.

Authors:  Robert Hasterok; John Draper; Glyn Jenkins
Journal:  Chromosome Res       Date:  2004       Impact factor: 5.239

2.  Analysis of chromosomal polymorphism in barley (Hordeum vulgare L. ssp. vulgare) and between H. vulgare and H. chilense using three-color fluorescence in situ hybridization (FISH).

Authors:  É Szakács; K Kruppa; M Molnár-Láng
Journal:  J Appl Genet       Date:  2013-08-29       Impact factor: 3.240

3.  Comparative analysis of rDNA distribution in chromosomes of various species of Brassicaceae.

Authors:  Robert Hasterok; Elzbieta Wolny; Marta Hosiawa; Malgorzata Kowalczyk; Sylwia Kulak-Ksiazczyk; Tomasz Ksiazczyk; Waheeb K Heneen; Jolanta Maluszynska
Journal:  Ann Bot       Date:  2005-12-15       Impact factor: 4.357

4.  Molecular cytogenetic analysis of Brassica rapa-Brassica oleracea var. alboglabra monosomic addition lines.

Authors:  Robert Hasterok; Elzbieta Wolny; Sylwia Kulak; Aleksandra Zdziechiewicz; Jolanta Maluszynska; Waheeb K Heneen
Journal:  Theor Appl Genet       Date:  2005-03-09       Impact factor: 5.699

5.  Multicolour FISH in an analysis of chromosome aberrations induced by N-nitroso-N-methylurea and maleic hydrazide in barley cells.

Authors:  Jolanta Juchimiuk; Barbara Hering; Jolanta Maluszynska
Journal:  J Appl Genet       Date:  2007       Impact factor: 3.240

6.  FISH in analysis of gamma ray-induced micronuclei formation in barley.

Authors:  Jolanta Juchimiuk-Kwasniewska; Lidia Brodziak; Jolanta Maluszynska
Journal:  J Appl Genet       Date:  2010-12-07       Impact factor: 3.240

7.  Painting the chromosomes of Brachypodium: current status and future prospects.

Authors:  Dominika Idziak; Alexander Betekhtin; Elzbieta Wolny; Karolina Lesniewska; Jonathan Wright; Melanie Febrer; Michael W Bevan; Glyn Jenkins; Robert Hasterok
Journal:  Chromosoma       Date:  2011-06-11       Impact factor: 4.316

8.  Brachypodium distachyon - A Useful Model in the Qualification of Mutagen-Induced Micronuclei Using Multicolor FISH.

Authors:  Arita Kus; Jolanta Kwasniewska; Robert Hasterok
Journal:  PLoS One       Date:  2017-01-24       Impact factor: 3.240

9.  Reconstructing the Evolution of Brachypodium Genomes Using Comparative Chromosome Painting.

Authors:  Alexander Betekhtin; Glyn Jenkins; Robert Hasterok
Journal:  PLoS One       Date:  2014-12-10       Impact factor: 3.240

10.  Alignment of the genomes of Brachypodium distachyon and temperate cereals and grasses using bacterial artificial chromosome landing with fluorescence in situ hybridization.

Authors:  Robert Hasterok; Agnieszka Marasek; Iain S Donnison; Ian Armstead; Ann Thomas; Ian P King; Elzbieta Wolny; Dominika Idziak; John Draper; Glyn Jenkins
Journal:  Genetics       Date:  2006-02-19       Impact factor: 4.562
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