Hongmei Zhu1, Hai Wen, Wanqing Liao. 1. Cyptoccucus Laboratory, Department of Dermatology, Changzheng Hospital Second Military Medical University, Shanghai 200003, China. hmzhu__cn@yahoo.com.cn
Abstract
OBJECTIVE: To observe the unique DNA profile and the relationship between DNA profile and phenotype of Trichophyton rubrum,and establish an effective molecular method to identify T. rubrum. METHODS: Three primers, including (GACA)(4), (GTG)(5) and M13 core sequence (5'-GAGGGTG-GCGGTTCT-3'), were used to distinguish variations among 20 clinical isolates of T. rubrum and Trichophyton mentagrophytes. RESULTS: Different PCR-fingerprinting was seen between T. rubrum and T. mentagrophytes using three different primers. 2 stains of T. rubrum were identified among 6 supposed T. mentagrophytes. CONCLUSIONS: T. rubrum can be distinguished using PCR, and (GACA)(4) is the most suitable primer.
OBJECTIVE: To observe the unique DNA profile and the relationship between DNA profile and phenotype of Trichophyton rubrum,and establish an effective molecular method to identify T. rubrum. METHODS: Three primers, including (GACA)(4), (GTG)(5) and M13 core sequence (5'-GAGGGTG-GCGGTTCT-3'), were used to distinguish variations among 20 clinical isolates of T. rubrum and Trichophyton mentagrophytes. RESULTS: Different PCR-fingerprinting was seen between T. rubrum and T. mentagrophytes using three different primers. 2 stains of T. rubrum were identified among 6 supposed T. mentagrophytes. CONCLUSIONS:T. rubrum can be distinguished using PCR, and (GACA)(4) is the most suitable primer.
Authors: M F Spesso; C T Nuncira; V L Burstein; D T Masih; M D Dib; L S Chiapello Journal: Eur J Clin Microbiol Infect Dis Date: 2013-02-15 Impact factor: 3.267