BACKGROUND: Cimetidine reverses immunosuppression following trauma, however, its effect on pure haemorrhagic shock is unknown. METHODS: Mice sensitized by injection of sheep red blood cells (SRBCs), were subjected to cardiac puncture and randomized to a control group-A (n=11) and three shock groups (35% of blood volume extracted): group-S had no treatment (n=16), group-CP received cimetidine 50mg/kg intraperitoneally (n=16), group-CW received oral cimetidine (200mg/kg per day, n=16). After 5 days, animals were challenged by injection of SRBCs into the foot-pad of the right hind paw (same volume of saline was injected into left paw). Foot-pad thickness ratios (FPTRs) were determined at 16 and 40 h, and inflammatory response was assessed histologically. RESULTS: At 16 h, FPTRs were greater in group-CW than group-S (P=0.01). There were no differences at 40 h. More animals in groups-CP and -CW had grade 3/4 inflammation, whilst group-S had the least inflammatory response (NS). CONCLUSIONS: Cimetidine prevents suppression of delayed hypersensitivity in this model.
BACKGROUND:Cimetidine reverses immunosuppression following trauma, however, its effect on pure haemorrhagic shock is unknown. METHODS:Mice sensitized by injection of sheep red blood cells (SRBCs), were subjected to cardiac puncture and randomized to a control group-A (n=11) and three shock groups (35% of blood volume extracted): group-S had no treatment (n=16), group-CP received cimetidine 50mg/kg intraperitoneally (n=16), group-CW received oral cimetidine (200mg/kg per day, n=16). After 5 days, animals were challenged by injection of SRBCs into the foot-pad of the right hind paw (same volume of saline was injected into left paw). Foot-pad thickness ratios (FPTRs) were determined at 16 and 40 h, and inflammatory response was assessed histologically. RESULTS: At 16 h, FPTRs were greater in group-CW than group-S (P=0.01). There were no differences at 40 h. More animals in groups-CP and -CW had grade 3/4 inflammation, whilst group-S had the least inflammatory response (NS). CONCLUSIONS:Cimetidine prevents suppression of delayed hypersensitivity in this model.
Authors: Marc P Hübner; David Larson; Marina N Torrero; Ellen Mueller; Yinghui Shi; Kristin E Killoran; Edward Mitre Journal: Clin Immunol Date: 2011-08-23 Impact factor: 3.969