Literature DB >> 12213232

Acyl-CoA Z9- and Z10-desaturase genes from a New Zealand leafroller moth species, Planotortrix octo.

G Hao1, W Liu, M O'Connor, W l Roelofs.   

Abstract

Two cDNAs encoding acyl-CoA Z9-desaturase from the fat body and Z10-desaturase from the pheromone gland of the greenhead leafroller moth, Planotortrix octo, were obtained by RACE PCR. The Z9-desaturase (Pocto-Z9) cDNA spans 2291 nt with an ORF encoding a 352 amino-acid protein, which has 65% identity to Trichoplusia ni Delta 9 desaturase (Tni-Z9). The Z10-desaturase (Pocto-Z10) cDNA spans 2777 nt with an ORF encoding a protein with 356 amino acids. Pocto-Z10 shows lower identity to Pocto-Z9 and Tni-Z9 (48 and 46%, respectively) and relatively higher identity to the Delta 11 desaturases of T. ni and Helicoverpa zea (57 and 56%, respectively). The ORFs of these two P. octo cDNAs were constructed into an expression vector, YEpOLEX, that complemented the unsaturated fatty acid (UFA) auxotrophy of a desaturase-deficient ole1 strain of Saccharomyces cerevisiae. Expression of Pocto-Z9 produced a 5:2 ratio of Z9-16 and Z9-18 acids, with minor amounts (<4%) of Z9-14, Z9-15, and Z9-17 acids. Pocto-Z10 was successfully expressed in the YEpOLEX system when complemented with Z11-18:Me, and the major desaturase product proved to be Z10-16:Acid. The results confirm the regio- and stereo-selectivity of this unusual Delta 10 desaturase.

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Year:  2002        PMID: 12213232     DOI: 10.1016/s0965-1748(01)00176-x

Source DB:  PubMed          Journal:  Insect Biochem Mol Biol        ISSN: 0965-1748            Impact factor:   4.714


  19 in total

1.  Evolution of moth sex pheromones via ancestral genes.

Authors:  Wendell L Roelofs; Weitian Liu; Guixia Hao; Hongmei Jiao; Alejandro P Rooney; Charles E Linn
Journal:  Proc Natl Acad Sci U S A       Date:  2002-09-17       Impact factor: 11.205

2.  A multifunctional desaturase involved in the biosynthesis of the processionary moth sex pheromone.

Authors:  Montserrat Serra; Benjamin Piña; José Luis Abad; Francisco Camps; Gemma Fabriàs
Journal:  Proc Natl Acad Sci U S A       Date:  2007-10-05       Impact factor: 11.205

3.  Molecular genetics and evolution of pheromone biosynthesis in Lepidoptera.

Authors:  Wendell L Roelofs; Alejandro P Rooney
Journal:  Proc Natl Acad Sci U S A       Date:  2003-07-22       Impact factor: 11.205

4.  Striking cuticular hydrocarbon dimorphism in the mason wasp Odynerus spinipes and its possible evolutionary cause (Hymenoptera: Chrysididae, Vespidae).

Authors:  Mareike Wurdack; Sina Herbertz; Daniel Dowling; Johannes Kroiss; Erhard Strohm; Hannes Baur; Oliver Niehuis; Thomas Schmitt
Journal:  Proc Biol Sci       Date:  2015-12-22       Impact factor: 5.349

5.  Sex pheromone evolution is associated with differential regulation of the same desaturase gene in two genera of leafroller moths.

Authors:  Jérôme Albre; Marjorie A Liénard; Tamara M Sirey; Silvia Schmidt; Leah K Tooman; Colm Carraher; David R Greenwood; Christer Löfstedt; Richard D Newcomb
Journal:  PLoS Genet       Date:  2012-01-26       Impact factor: 5.917

6.  Transcriptome comparison of the sex pheromone glands from two sibling Helicoverpa species with opposite sex pheromone components.

Authors:  Zhao-Qun Li; Shuai Zhang; Jun-Yu Luo; Chun-Yi Wang; Li-Min Lv; Shuang-Lin Dong; Jin-Jie Cui
Journal:  Sci Rep       Date:  2015-03-20       Impact factor: 4.379

7.  Genes involved in sex pheromone biosynthesis of Ephestia cautella, an important food storage pest, are determined by transcriptome sequencing.

Authors:  Binu Antony; Alan Soffan; Jernej Jakše; Sulieman Alfaifi; Koko D Sutanto; Saleh A Aldosari; Abdulrahman S Aldawood; Arnab Pain
Journal:  BMC Genomics       Date:  2015-07-18       Impact factor: 3.969

8.  Key biosynthetic gene subfamily recruited for pheromone production prior to the extensive radiation of Lepidoptera.

Authors:  Marjorie A Liénard; Maria Strandh; Erik Hedenström; Tomas Johansson; Christer Löfstedt
Journal:  BMC Evol Biol       Date:  2008-10-02       Impact factor: 3.260

9.  Characterization of Z/E11- and Z9-desaturases from the obliquebanded leafroller moth, Choristoneura rosaceana.

Authors:  Guixia Hao; Marion O'Connor; Weitian Liu; Wendell L Roelofs
Journal:  J Insect Sci       Date:  2002-12-17       Impact factor: 1.857

10.  Analysis of the Agrotis segetum pheromone gland transcriptome in the light of sex pheromone biosynthesis.

Authors:  Bao-Jian Ding; Christer Löfstedt
Journal:  BMC Genomics       Date:  2015-09-18       Impact factor: 3.969

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