Sex- and age-related elevation of cochlear nerve envelope response (CNER) and auditory brainstem response (ABR) thresholds in C57BL/6 mice.

The C57BL/6 mouse has long been considered, in scores of published studies, as a model of early adult-onset, progressive sensorineural hearing loss (presbycusis). The auditory brainstem response (ABR) has most often been used in these studies as a measure of functional loss. Whereas the ABR measures the response to a rapid acoustic onset, the cochlear nerve envelope response (CNER) measures the ability of cochlear nerve axons to respond to the low frequency modulations of the entire acoustic waveform, acoustic changes that are utilized in vocalizations and music. The present study compared the ability of these two measures to assess presbycusis in male and female C57BL/6 mice, at ages ranging from 50 to 400 days. Thresholds to the CNER were almost invariably more sensitive than the ABR, in response to stimulus frequencies ranging from 8 to 56 kHz. By 100 days of age, mice showed elevation of thresholds in response to high frequency stimuli, and this loss was greater in females than in males. These trends persisted for both measures over the next 300 days, involving successively lower frequencies.