| Literature DB >> 12202992 |
Nobuyo Yoshida1, Yuko Nishimaki, Masahide Sugiyama, Takashi Abe, Taiga Tatsumi, Akito Tanoue, Akira Hirasawa, Gozoh Tsujimoto.
Abstract
The beta(2)-adrenergic receptor (beta2AR) is the key target for the beta(2)-agonist drugs used for bronchodilation in asthma and chronic obstructive pulmonary disease. To detect four SNPs with amino acid variations at positions -47T/C (CysBUP19Arg), 46A/G (Gly16Arg), 79C/G (Gln27Glu), and 491C/T (Thr164Ile) in the beta 2AR gene, we used the electronic microchip assay, denaturing high-performance liquid chromatography (DHPLC), and direct sequencing. Genomic DNA samples were obtained from the blood of 84 Japanese healthy volunteers. The agreement rates of the first data set with the final data (allele calls) were 99.7% (332/333), 99.2% (246/248), and 96.7% (329/340). The percentages of no allele designation (ND) were 2.06% (7/340), 2.75% (7/255), and 0.00% (0/340) for the electronic microchip assay, DHPLC, and direct sequencing, respectively. Furthermore, we found three samples that had a novel haplotype.Entities:
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Year: 2002 PMID： 12202992 DOI： 10.1007/s100380200074
Source DB: PubMed Journal: J Hum Genet ISSN： 1434-5161 Impact factor: 3.172