Literature DB >> 12202605

Comparison of serological detection methods for diagnosis of Ehrlichia canis infections in dogs.

Myriam Bélanger1, Heather L Sorenson, Michelle K France, Michael V Bowie, Anthony F Barbet, Edward B Breitschwerdt, A Rick Alleman.   

Abstract

We determined the value of four serological assays for the diagnosis of canine monocytic ehrlichiosis by comparing them to the indirect fluorescent-antibody assay "gold standard." The specificity of Dip-S-Ticks was significantly lower than that of all of the other tests evaluated. The sensitivity of Dip-S-Ticks was significantly higher than that of Snap3Dx or the Snap Canine Combo. The sensitivity of the rMAP2 enzyme-linked immunosorbent assay (ELISA) was significantly higher than that of the Snap Canine Combo. The accuracy levels of the rMAP2 ELISA, Snap3Dx, Dip-S-Ticks, and Snap Canine Combo were 97.0, 89.8, 85.1, and 82.9%, respectively.

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Year:  2002        PMID： 12202605      PMCID： PMC130819          DOI： 10.1128/JCM.40.9.3506-3508.2002

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  13 in total

1.  Comparison of a clinic-based ELISA test kit with the immunofluorescence test for the assay of Ehrlichia canis antibodies in dogs.

Authors:  T Waner; C Strenger; A Keysary
Journal:  J Vet Diagn Invest       Date:  2000-05       Impact factor: 1.279

Review 2.  Significance of serological testing for ehrlichial diseases in dogs with special emphasis on the diagnosis of canine monocytic ehrlichiosis caused by Ehrlichia canis.

Authors:  T Waner; S Harrus; F Jongejan; H Bark; A Keysary; A W Cornelissen
Journal:  Vet Parasitol       Date:  2001-02       Impact factor: 2.738

3.  Immunodiagnosis of Ehrlichia canis infection with recombinant proteins.

Authors:  J W McBride; R E Corstvet; E B Breitschwerdt; D H Walker
Journal:  J Clin Microbiol       Date:  2001-01       Impact factor: 5.948

4.  Western immunoblotting analysis of the antibody responses of patients with human monocytotropic ehrlichiosis to different strains of Ehrlichia chaffeensis and Ehrlichia canis.

Authors:  S M Chen; L C Cullman; D H Walker
Journal:  Clin Diagn Lab Immunol       Date:  1997-11

5.  Coinfection with multiple tick-borne pathogens in a Walker Hound kennel in North Carolina.

Authors:  S K Kordick; E B Breitschwerdt; B C Hegarty; K L Southwick; C M Colitz; S I Hancock; J M Bradley; R Rumbough; J T Mcpherson; J N MacCormack
Journal:  J Clin Microbiol       Date:  1999-08       Impact factor: 5.948

6.  Doxycycline hyclate treatment of experimental canine ehrlichiosis followed by challenge inoculation with two Ehrlichia canis strains.

Authors:  E B Breitschwerdt; B C Hegarty; S I Hancock
Journal:  Antimicrob Agents Chemother       Date:  1998-02       Impact factor: 5.191

7.  Cloning and characterization of multigenes encoding the immunodominant 30-kilodalton major outer membrane proteins of Ehrlichia canis and application of the recombinant protein for serodiagnosis.

Authors:  N Ohashi; A Unver; N Zhi; Y Rikihisa
Journal:  J Clin Microbiol       Date:  1998-09       Impact factor: 5.948

8.  Sequential evaluation of dogs naturally infected with Ehrlichia canis, Ehrlichia chaffeensis, Ehrlichia equi, Ehrlichia ewingii, or Bartonella vinsonii.

Authors:  E B Breitschwerdt; B C Hegarty; S I Hancock
Journal:  J Clin Microbiol       Date:  1998-09       Impact factor: 5.948

9.  Molecular characterization of a 28 kDa surface antigen gene family of the tribe Ehrlichiae.

Authors:  G R Reddy; C R Sulsona; A F Barbet; S M Mahan; M J Burridge; A R Alleman
Journal:  Biochem Biophys Res Commun       Date:  1998-06-29       Impact factor: 3.575

10.  Western immunoblot analysis of Ehrlichia chaffeensis, E. canis, or E. ewingii infections in dogs and humans.

Authors:  Y Rikihisa; S A Ewing; J C Fox
Journal:  J Clin Microbiol       Date:  1994-09       Impact factor: 5.948
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  7 in total

1.  Multiplex detection of Ehrlichia and Anaplasma species pathogens in peripheral blood by real-time reverse transcriptase-polymerase chain reaction.

Authors:  Kamesh R Sirigireddy; Roman R Ganta
Journal:  J Mol Diagn       Date:  2005-05       Impact factor: 5.568

2.  Enzyme-linked immunosorbent assay with conserved immunoreactive glycoproteins gp36 and gp19 has enhanced sensitivity and provides species-specific immunodiagnosis of Ehrlichia canis infection.

Authors:  Ana Maria Cárdenas; C Kuyler Doyle; Xiaofeng Zhang; Kimberly Nethery; Richard E Corstvet; David H Walker; Jere W McBride
Journal:  Clin Vaccine Immunol       Date:  2006-12-06

3.  Frequency and Clinical Epidemiology of Canine Monocytic Ehrlichiosis in Dogs Infested with Ticks from Sinaloa, Mexico.

Authors:  Carolina Guadalupe Sosa-Gutierrez; Maria Teresa Quintero Martinez; Soila Maribel Gaxiola Camacho; Silvia Cota Guajardo; Maria D Esteve-Gassent; María-Guadalupe Gordillo-Pérez
Journal:  J Vet Med       Date:  2013-10-24

4.  A new TaqMan method for the reliable diagnosis of Ehrlichia spp. in canine whole blood.

Authors:  Kirsty Thomson; Tal Yaaran; Alex Belshaw; Lucia Curson; Laurence Tisi; Sarah Maurice; Guy Kiddle
Journal:  Parasit Vectors       Date:  2018-06-18       Impact factor: 3.876

5.  A newly developed droplet digital PCR for Ehrlichia canis detection: comparisons to conventional PCR and blood smear techniques.

Authors:  Sakulchit Wichianchot; Nuttanan Hongsrichan; Cherdsak Maneeruttanarungroj; Somchai Pinlaor; Kantapong Iamrod; Andaman Purisarn; Peerawich Donthaisong; Panagiotis Karanis; Burin Nimsuphan; Rucksak Rucksaken
Journal:  J Vet Med Sci       Date:  2022-04-27       Impact factor: 1.105

6.  Ehrlichiosis, babesiosis, anaplasmosis and hepatozoonosis in dogs from St. Kitts, West Indies.

Authors:  Patrick J Kelly; Chuanling Xu; Helene Lucas; Amanda Loftis; Jamie Abete; Frank Zeoli; Audrey Stevens; Kirsten Jaegersen; Kate Ackerson; April Gessner; Bernhard Kaltenboeck; Chengming Wang
Journal:  PLoS One       Date:  2013-01-15       Impact factor: 3.240

7.  Demonstrating the presence of Ehrlichia canis DNA from different tissues of dogs with suspected subclinical ehrlichiosis.

Authors:  Carlos A Rodríguez-Alarcón; Diana M Beristain-Ruiz; Angélica Olivares-Muñoz; Andrés Quezada-Casasola; Federico Pérez-Casio; Jesús A Álvarez-Martínez; Jane Tapia-Alanís; José J Lira-Amaya; Ramón Rivera-Barreno; Orlando S Cera-Hurtado; José A Ibancovichi-Camarillo; Luis Soon-Gómez; Jaime R Adame-Gallegos; Julio V Figueroa-Millán
Journal:  Parasit Vectors       Date:  2020-10-15       Impact factor: 3.876
  7 in total

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