Literature DB >> 12202602

Technical improvement to prevent DNA degradation of enteric pathogens in pulsed-field gel electrophoresis.

Joanna M K Koort1, Susanna Lukinmaa, Marjatta Rantala, Erja Unkila, Anja Siitonen.   

Abstract

This study used a modified pulsed-field gel electrophoresis (PFGE) method with HEPES as a running buffer to prevent electrophoresis-related DNA degradation of nine Salmonella enterica subsp. enterica serovar Ohio, seven Salmonella serovar Newport, and two enterohemorrhagic Escherichia coli (non-O157) strains. All strains yielded identifiable bands with this method in contrast to a commonly applied PFGE method using Tris buffer.

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Year:  2002        PMID： 12202602      PMCID： PMC130692          DOI： 10.1128/JCM.40.9.3497-3498.2002

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  12 in total

1.  Diversity of strains of Salmonella enterica serotype enteritidis from English poultry farms assessed by multiple genetic fingerprinting.

Authors:  E Liebana; L Garcia-Migura; M F Breslin; R H Davies; M J Woodward
Journal:  J Clin Microbiol       Date:  2001-01       Impact factor: 5.948

2.  Achieving 100% typeability of Pseudomonas aeruginosa by pulsed-field gel electrophoresis.

Authors:  U Römling; B Tümmler
Journal:  J Clin Microbiol       Date:  2000-01       Impact factor: 5.948

3.  Pulsed-field gel electrophoresis of degradation-sensitive DNAs from Clostridium difficile PCR ribotype 1 strains.

Authors:  J E Corkill; R Graham; C A Hart; S Stubbs
Journal:  J Clin Microbiol       Date:  2000-07       Impact factor: 5.948

4.  Tris-dependent site-specific cleavage of Streptomyces lividans DNA.

Authors:  T Ray; J Weaden; P Dyson
Journal:  FEMS Microbiol Lett       Date:  1992-09-15       Impact factor: 2.742

5.  Rapid pulsed-field gel electrophoresis protocol for typing of Escherichia coli O157:H7 and other gram-negative organisms in 1 day.

Authors:  R K Gautom
Journal:  J Clin Microbiol       Date:  1997-11       Impact factor: 5.948

6.  High-resolution genotyping of Salmonella strains by AFLP-fingerprinting.

Authors:  H J Aarts; L A van Lith; J Keijer
Journal:  Lett Appl Microbiol       Date:  1998-02       Impact factor: 2.858

7.  Salmonella enteritidis phage types 1 and 4: pheno- and genotypic epidemiology of recent outbreaks in Finland.

Authors:  S Lukinmaa; R Schildt; T Rinttilä; A Siitonen
Journal:  J Clin Microbiol       Date:  1999-07       Impact factor: 5.948

8.  Fluorescent amplified-fragment length polymorphism subtyping of the Salmonella enterica serovar enteritidis phage type 4 clone complex.

Authors:  M Desai; E J Threlfall; J Stanley
Journal:  J Clin Microbiol       Date:  2001-01       Impact factor: 5.948

9.  Molecular epidemiology of an outbreak caused by Salmonella enterica serovar Newport in Finland and the United Kingdom.

Authors:  O Lyytikäinen; J Koort; L Ward; R Schildt; P Ruutu; E Japisson; M Timonen; A Siitonen
Journal:  Epidemiol Infect       Date:  2000-04       Impact factor: 2.451

10.  Evaluation of DNA fingerprinting by PFGE as an epidemiologic tool for Salmonella infections.

Authors:  T Murase; T Okitsu; R Suzuki; H Morozumi; A Matsushima; A Nakamura; S Yamai
Journal:  Microbiol Immunol       Date:  1995       Impact factor: 1.955
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  20 in total

1.  rpoS-Regulated core genes involved in the competitive fitness of Salmonella enterica Serovar Kentucky in the intestines of chickens.

Authors:  Ying Cheng; Adriana Ayres Pedroso; Steffen Porwollik; Michael McClelland; Margie D Lee; Tiffany Kwan; Katherine Zamperini; Vivek Soni; Holly S Sellers; Scott M Russell; John J Maurer
Journal:  Appl Environ Microbiol       Date:  2014-10-31       Impact factor: 4.792

2.  Insertions, deletions, and single-nucleotide polymorphisms at rare restriction enzyme sites enhance discriminatory power of polymorphic amplified typing sequences, a novel strain typing system for Escherichia coli O157:H7.

Authors:  Indira T Kudva; Robert W Griffin; Megan Murray; Manohar John; Nicole T Perna; Timothy J Barrett; Stephen B Calderwood
Journal:  J Clin Microbiol       Date:  2004-06       Impact factor: 5.948

3.  Comparison of a PCR-restriction fragment length polymorphism (PCR-RFLP) assay to pulsed-field gel electrophoresis to determine the effect of repeated subculture and prolonged storage on RFLP patterns of Shiga toxin-producing Escherichia coli O157:H7.

Authors:  Kensuke Shima; Yuluo Wu; Norihiko Sugimoto; Masahiro Asakura; Kazuhiko Nishimura; Shinji Yamasaki
Journal:  J Clin Microbiol       Date:  2006-09-13       Impact factor: 5.948

4.  Tracing shigatoxigenic Escherichia coli O103, O145, and O174 infections from farm residents to cattle.

Authors:  Sirpa Heinikainen; Tarja Pohjanvirta; Marjut Eklund; Anja Siitonen; Sinikka Pelkonen
Journal:  J Clin Microbiol       Date:  2007-09-05       Impact factor: 5.948

5.  Comparative Genomic Analysis of Third-Generation-Cephalosporin-Resistant Escherichia coli Harboring the bla CMY-2-Positive IncI1 Group, IncB/O/K/Z, and IncC Plasmids Isolated from Healthy Broilers in Japan.

Authors:  Takahiro Shirakawa; Tsuyoshi Sekizuka; Makoto Kuroda; Satowa Suzuki; Manao Ozawa; Hitoshi Abo; Yukari Furuya; Ryoko Akama; Mari Matsuda; Yoko Shimazaki; Mayumi Kijima; Michiko Kawanishi
Journal:  Antimicrob Agents Chemother       Date:  2020-06-23       Impact factor: 5.191

6.  Development of a PCR-restriction fragment length polymorphism assay for the epidemiological analysis of Shiga toxin-producing Escherichia coli.

Authors:  Kensuke Shima; Jun Terajima; Toshio Sato; Kazuhiko Nishimura; Kazumichi Tamura; Haruo Watanabe; Yoshifumi Takeda; Shinji Yamasaki
Journal:  J Clin Microbiol       Date:  2004-11       Impact factor: 5.948

7.  Verocytotoxin-producing Escherichia coli in wild birds and rodents in close proximity to farms.

Authors:  Eva Møller Nielsen; Marianne N Skov; Jesper J Madsen; Jens Lodal; Jørgen Brøchner Jespersen; Dorte L Baggesen
Journal:  Appl Environ Microbiol       Date:  2004-11       Impact factor: 4.792

8.  Variant Salmonella genomic island 1 antibiotic resistance gene cluster containing a novel 3'-N-aminoglycoside acetyltransferase gene cassette, aac(3)-Id, in Salmonella enterica serovar newport.

Authors:  Benoît Doublet; François-Xavier Weill; Laetitia Fabre; Elisabeth Chaslus-Dancla; Axel Cloeckaert
Journal:  Antimicrob Agents Chemother       Date:  2004-10       Impact factor: 5.191

9.  Epidemiology of a Salmonella enterica subsp. enterica serovar Typhimurium strain associated with a songbird outbreak.

Authors:  Sonia M Hernandez; Kevin Keel; Susan Sanchez; Eija Trees; Peter Gerner-Smidt; Jennifer K Adams; Ying Cheng; Al Ray; Gordon Martin; Andrea Presotto; Mark G Ruder; Justin Brown; David S Blehert; Walter Cottrell; John J Maurer
Journal:  Appl Environ Microbiol       Date:  2012-08-10       Impact factor: 4.792

10.  Pulsed-field gel electrophoresis analysis of Vibrio vulnificus strains isolated from Taiwan and the United States.

Authors:  Hin-Chung Wong; Shau-Yan Chen; Meng-Yi Chen; James D Oliver; Lien-I Hor; Wen-Cherng Tsai
Journal:  Appl Environ Microbiol       Date:  2004-09       Impact factor: 4.792
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