Literature DB >> 12199141

Cytogenetic characterization of telomeres in the holocentric chromosomes of the lepidopteran Mamestra brassicae.

M Mandrioli1.   

Abstract

Telomeres of the Mamestra brassica holocentric chromosomes were studied by Southern blotting, in-situ hybridization and Bal31 assay evidencing the presence of the telomeric (TTAGG)n repeat. Successively, molecular analysis of telomeres showed that TRAS1 transposable elements were present at the subtelomeric regions of autosomes but not in the NOR-bearing telomeres of the Z and W sex chromosomes. TRAS1 appeared to be transcriptionally active and non-methylated, as evaluated by RT-PCR and digestion with MspI and HpaII. Finally, dot-blotting experiments showed that the 2.8 +/- 0.5% of the M. brassicae genome consists of TRAS1.

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Year:  2002        PMID： 12199141     DOI： 10.1023/a:1016515607278

Source DB:  PubMed          Journal:  Chromosome Res        ISSN： 0967-3849            Impact factor:   4.620


  29 in total

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