Wei Zhu1, Yuliang Ma, George B Stefano. 1. Neuroscience Research Institute, State University of New York, College at Old Westbury, Old Westbury, NY 11568, USA.
Abstract
UNLABELLED: Tetrahydropapaveroline and reticuline, isoquinoline alkaloids, were purified and identified in the pedal ganglia of the marine mollusk Mytilus edulis. OBJECTIVES: To determine the presence of tetrahydropapaveroline and reticuline, isoquinoline alkaloids, in animal tissues previously shown to express opiate receptors. METHODS AND RESULTS: This was achieved by high performance liquid chromatography coupled with electrochemical detection and finally identified by electro-spray ionization quadrupole time of flight mass spectrometry (Q-TOF). The expression of these two terahydroisoquinoline alkaloids in the animals neural tissue is at the level of 2.5+/-0.64 ng/ganglion and 1.8+/-0.52 ng/ganglion, respectively. CONCLUSION: This finding suggests that morphine biosynthesis may occur in Mytilus neural tissues, and that the biosynthesis pathway may be similar to that reported in the poppy plant.
UNLABELLED: Tetrahydropapaveroline and reticuline, isoquinoline alkaloids, were purified and identified in the pedal ganglia of the marine mollusk Mytilus edulis. OBJECTIVES: To determine the presence of tetrahydropapaveroline and reticuline, isoquinoline alkaloids, in animal tissues previously shown to express opiate receptors. METHODS AND RESULTS: This was achieved by high performance liquid chromatography coupled with electrochemical detection and finally identified by electro-spray ionization quadrupole time of flight mass spectrometry (Q-TOF). The expression of these two terahydroisoquinoline alkaloids in the animals neural tissue is at the level of 2.5+/-0.64 ng/ganglion and 1.8+/-0.52 ng/ganglion, respectively. CONCLUSION: This finding suggests that morphine biosynthesis may occur in Mytilus neural tissues, and that the biosynthesis pathway may be similar to that reported in the poppy plant.
Authors: George B Stefano; Richard M Kream; Kirk J Mantione; Melinda Sheehan; Patrick Cadet; Wei Zhu; Thomas V Bilfinger; Tobias Esch Journal: Semin Cancer Biol Date: 2007-12-08 Impact factor: 15.707
Authors: George B Stefano; Kirk J Mantione; Milena Králíčková; Radek Ptacek; Hana Kuzelova; Tobias Esch; Richard M Kream Journal: Med Sci Monit Date: 2012-08