BACKGROUND/ PURPOSE: It was believed previously that pulmonary hypoplasia in congenital diaphragmatic hernia (CDH) was a consequence of the herniation of abdominal viscera into the chest. Using the murine nitrofen-induced model of CDH, the authors evaluated lung growth and development before diaphragm closure or herniation. METHODS: The authors examined nitrofen-exposed early embryonic lungs on embryonic day 12 (E12). Branching morphogenesis was quantified before and after 4 days in culture in serumless chemically defined media and compared with age-matched control lungs. The mRNA expression of proliferative and developmental markers in cultured lungs was then determined. RESULTS: Nitrofen-exposed lungs had 30% fewer total terminal branches than age-matched controls (9.3 +/- 1.9 nitrofen v 13.7 +/- 2.6 control; P <.001). Hypoplasia also was more profound in the left than the right lung. These effects persisted after culturing the lungs for 4 days in serumless chemically-defined media (31.7 +/- 6.8 nitrofen v 42.9 +/- 8.4 control, P <.001). Furthermore, the mRNA expression of proliferative and developmental markers was decreased in nitrofen-exposed E12 lungs cultured for 4 days (as a percentage of age-matched controls): cyclin A (69.28%; P =.04), Nkx2.1 (44.4%, 0.04), SP-A (24.1%; P =.008), SP-B (23.4%; P =.05), SP-C (20%; P =.06), and CC-10 (13.8%; P =.04). CONCLUSION: Nitrofen induces primary pulmonary hypoplasia and immaturity in the early embryonic mouse, and this effect persists in culture. Copyright 2002, Elsevier Science (USA). All rights reserved.
BACKGROUND/ PURPOSE: It was believed previously that pulmonary hypoplasia in congenital diaphragmatic hernia (CDH) was a consequence of the herniation of abdominal viscera into the chest. Using the murinenitrofen-induced model of CDH, the authors evaluated lung growth and development before diaphragm closure or herniation. METHODS: The authors examined nitrofen-exposed early embryonic lungs on embryonic day 12 (E12). Branching morphogenesis was quantified before and after 4 days in culture in serumless chemically defined media and compared with age-matched control lungs. The mRNA expression of proliferative and developmental markers in cultured lungs was then determined. RESULTS:Nitrofen-exposed lungs had 30% fewer total terminal branches than age-matched controls (9.3 +/- 1.9 nitrofen v 13.7 +/- 2.6 control; P <.001). Hypoplasia also was more profound in the left than the right lung. These effects persisted after culturing the lungs for 4 days in serumless chemically-defined media (31.7 +/- 6.8 nitrofen v 42.9 +/- 8.4 control, P <.001). Furthermore, the mRNA expression of proliferative and developmental markers was decreased in nitrofen-exposed E12 lungs cultured for 4 days (as a percentage of age-matched controls): cyclin A (69.28%; P =.04), Nkx2.1 (44.4%, 0.04), SP-A (24.1%; P =.008), SP-B (23.4%; P =.05), SP-C (20%; P =.06), and CC-10 (13.8%; P =.04). CONCLUSION:Nitrofen induces primary pulmonary hypoplasia and immaturity in the early embryonic mouse, and this effect persists in culture. Copyright 2002, Elsevier Science (USA). All rights reserved.
Authors: Todd Jensen; Blair Roszell; Fan Zang; Eric Girard; Adam Matson; Roger Thrall; Diane M Jaworski; Cayla Hatton; Daniel J Weiss; Christine Finck Journal: Tissue Eng Part C Methods Date: 2012-04-17 Impact factor: 3.056
Authors: Nelson LopezJimenez; Simon Gerber; Vlad Popovici; Sonia Mirza; Kirsten Copren; Linda Ta; Gary M Shaw; Beat Trueb; Anne M Slavotinek Journal: Hum Genet Date: 2009-12-19 Impact factor: 4.132
Authors: M Cecilia Crisanti; Sirma H Koutzaki; Mark J Mondrinos; Peter I Lelkes; Christine M Finck Journal: J Surg Res Date: 2007-07-25 Impact factor: 2.192
Authors: Shaun M Kunisaki; Guihua Jiang; Juan C Biancotti; Kenneth K Y Ho; Briana R Dye; Allen P Liu; Jason R Spence Journal: Stem Cells Transl Med Date: 2020-09-19 Impact factor: 6.940