AIM: The purpose of this study was to determine whether AH 26 and AH Plus have in vitro oestrogenic effects. METHODOLOGY: MCF-7 breast cancer cells were trypsinized and plated in 24-well plates at initial concentrations of 10,000 cells per well in 5% FBS in DME. Cells were allowed to attach for 24 h; then the seeding medium was replaced with 10% CDHuS-supplemented phenol red-free DME. Different concentrations of the test compound were added to sample wells (AH 26 and AH Plus at 1/100 to 1/1,000,000 dilutions), 10 pM estradiol-17beta to positive controL and no sub-stance to negative control (hormone-free control cells). The assay was stopped after 144 h. RESULTS: AH 26-powder induced MCF-7 cell proliferation in a dose-dependent manner. The cell yield obtained with AH 26-powder at 1/100 dilution sample was 2.5-fold greater than in control cultures. The sam-ple prepared with mixed AH 26 paste/powder 1:1 also induced MCF-7 cell proliferation, but showed less potency than AH 26-powder alone. The cell yield obtained with AH 26 paste/powder at 1/100 dilution sample was 1.9-fold greater than in control cultures. AH Plus did not show in vitro oestrogenic effect. CONCLUSIONS: AH 26 showed in vitro oestrogenic effect, but not AH Plus. The endodontist must consider the possible oestrogenic effect of AH 26, as well as the cytotoxic effects of root filling materials, and avoid the leakage of sealer through the apex during root canal treatment.
AIM: The purpose of this study was to determine whether AH 26 and AH Plus have in vitro oestrogenic effects. METHODOLOGY: MCF-7 breast cancer cells were trypsinized and plated in 24-well plates at initial concentrations of 10,000 cells per well in 5% FBS in DME. Cells were allowed to attach for 24 h; then the seeding medium was replaced with 10% CDHuS-supplemented phenol red-free DME. Different concentrations of the test compound were added to sample wells (AH 26 and AH Plus at 1/100 to 1/1,000,000 dilutions), 10 pM estradiol-17beta to positive controL and no sub-stance to negative control (hormone-free control cells). The assay was stopped after 144 h. RESULTS: AH 26-powder induced MCF-7 cell proliferation in a dose-dependent manner. The cell yield obtained with AH 26-powder at 1/100 dilution sample was 2.5-fold greater than in control cultures. The sam-ple prepared with mixed AH 26 paste/powder 1:1 also induced MCF-7 cell proliferation, but showed less potency than AH 26-powder alone. The cell yield obtained with AH 26 paste/powder at 1/100 dilution sample was 1.9-fold greater than in control cultures. AH Plus did not show in vitro oestrogenic effect. CONCLUSIONS: AH 26 showed in vitro oestrogenic effect, but not AH Plus. The endodontist must consider the possible oestrogenic effect of AH 26, as well as the cytotoxic effects of root filling materials, and avoid the leakage of sealer through the apex during root canal treatment.