Literature DB >> 12189842

Probing the effect of a 3'-S-phosphorothiolate link on the conformation of a DNA:RNA hybrid; implications for antisense drug design.

Andrew P G Beevers1, Kevin J Fettes, Ian A O'Neil, Stanley M Roberts, John R P Arnold, Richard Cosstick, Julie Fisher.   

Abstract

The effects of a single 3'-S-phosphorothiolate link in the DNA strand of a DNA:RNA dodecamer duplex is described; the sulfur induces a conformational shift in the (attached) sugar pucker, as shown by 1H NMR studies, and increases the thermal stability of the duplex compared to the non-modified system.

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Year:  2002        PMID： 12189842     DOI： 10.1039/b203582k

Source DB:  PubMed          Journal:  Chem Commun (Camb)        ISSN： 1359-7345            Impact factor:   6.222


  4 in total

1.  Synthesis of phosphorothioamidites derived from 3'-thio-3'-deoxythymidine and 3'-thio-2',3'-dideoxycytidine and the automated synthesis of oligodeoxynucleotides containing a 3'-S-phosphorothiolate linkage.

Authors:  Ghalia Sabbagh; Kevin J Fettes; Rajendra Gosain; Ian A O'Neil; Richard Cosstick
Journal:  Nucleic Acids Res       Date:  2004-01-23       Impact factor: 16.971

2.  A general and efficient approach for the construction of RNA oligonucleotides containing a 5'-phosphorothiolate linkage.

Authors:  Nan-Sheng Li; John K Frederiksen; Selene C Koo; Jun Lu; Timothy J Wilson; David M J Lilley; Joseph A Piccirilli
Journal:  Nucleic Acids Res       Date:  2010-12-09       Impact factor: 16.971

3.  Synthesis and biochemical application of 2'-O-methyl-3'-thioguanosine as a probe to explore group I intron catalysis.

Authors:  Jun Lu; Nan-Sheng Li; Raghuvir N Sengupta; Joseph A Piccirilli
Journal:  Bioorg Med Chem       Date:  2008-03-27       Impact factor: 3.641

4.  In vitro and in vivo properties of therapeutic oligonucleotides containing non-chiral 3' and 5' thiophosphate linkages.

Authors:  Jörg Duschmalé; Henrik Frydenlund Hansen; Martina Duschmalé; Erich Koller; Nanna Albaek; Marianne Ravn Møller; Klaus Jensen; Troels Koch; Jesper Wengel; Konrad Bleicher
Journal:  Nucleic Acids Res       Date:  2020-01-10       Impact factor: 16.971
  4 in total

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