OBJECTIVE: To investigate associations of polymorphisms in the LEP, LEPR and NPY genes with obesity-related traits in a Brazilian population of European descent. METHODS: A total of 183 women and 153 men (mean body mass index (BMI), 26.1+/-4.8 kg/m(2)) were genotyped using the PCR-RFLP procedure for the LEP A19G, LEPR Gln223Arg, LEPR PRO1019pro and NPY Leu7Pro polymorphisms. Frequencies were compared among normal-weight and overweight plus obese groups with chi-square tests, mean BMI and waist circumference were compared among genotypes by t-tests and analysis of variance. RESULTS: The genotype and allele frequencies of the LEPR Gln223Arg polymorphism were significantly different between normal-weight and overweight plus obese groups (P=0.013 and 0.009, respectively). Although there was no difference in the mean adjusted BMI among the three LEPRGln223Arg genotypes, a trend was observed for Arg/Arg individuals to have a higher mean BMI compared to Gln/Gln homozygotes, with heterozygote individuals presenting intermediate mean BMI between the two homozygote groups (ANOVA, P=0.063). However, in non-smokers, the LEPR Gln223Arg polymorphism showed a highly significant effect over BMI (P=0.009). When the analysis was restricted to premenopausal women, a highly significant effect of NPY was observed. Women bearing the Pro variant presented a lower BMI than wild-type homozygotes (P=0.001). CONCLUSION: Our findings suggest that genetic variability in the leptin receptor and neuropeptide Y genes is implicated in body weight regulation, the LEPR Gln223Arg variant being associated with a BMI increase in this Caucasian population, especially in non-smokers, while the NPY Leu7Pro polymorphism was associated with BMI reduction in premenopausal women.
OBJECTIVE: To investigate associations of polymorphisms in the LEP, LEPR and NPY genes with obesity-related traits in a Brazilian population of European descent. METHODS: A total of 183 women and 153 men (mean body mass index (BMI), 26.1+/-4.8 kg/m(2)) were genotyped using the PCR-RFLP procedure for the LEPA19G, LEPRGln223Arg, LEPR PRO1019pro and NPYLeu7Pro polymorphisms. Frequencies were compared among normal-weight and overweight plus obese groups with chi-square tests, mean BMI and waist circumference were compared among genotypes by t-tests and analysis of variance. RESULTS: The genotype and allele frequencies of the LEPRGln223Arg polymorphism were significantly different between normal-weight and overweight plus obese groups (P=0.013 and 0.009, respectively). Although there was no difference in the mean adjusted BMI among the three LEPRGln223Arg genotypes, a trend was observed for Arg/Arg individuals to have a higher mean BMI compared to Gln/Gln homozygotes, with heterozygote individuals presenting intermediate mean BMI between the two homozygote groups (ANOVA, P=0.063). However, in non-smokers, the LEPRGln223Arg polymorphism showed a highly significant effect over BMI (P=0.009). When the analysis was restricted to premenopausal women, a highly significant effect of NPY was observed. Women bearing the Pro variant presented a lower BMI than wild-type homozygotes (P=0.001). CONCLUSION: Our findings suggest that genetic variability in the leptin receptor and neuropeptide Y genes is implicated in body weight regulation, the LEPRGln223Arg variant being associated with a BMI increase in this Caucasian population, especially in non-smokers, while the NPYLeu7Pro polymorphism was associated with BMI reduction in premenopausal women.
Authors: Shruti Dasgupta; Mohammed Salman; Lokesh B Siddalingaiah; G L Lakshmi; D Xaviour; Jwalapuram Sreenath Journal: Adipocyte Date: 2014-12-20 Impact factor: 4.534
Authors: George Stratigopoulos; Charles A LeDuc; Naoki Matsuoka; Roee Gutman; Richard Rausch; Scott A Robertson; Martin G Myers; Wendy K Chung; Streamson C Chua; Rudolph L Leibel Journal: Obesity (Silver Spring) Date: 2008-11-06 Impact factor: 5.002
Authors: Belinda K Cornes; Sarah E Medland; Penelope A Lind; Dale R Nyholt; Grant W Montgomery; Nicholas G Martin Journal: Twin Res Hum Genet Date: 2009-06 Impact factor: 1.587
Authors: Marco Aurélio Echart Montano; Juan Pablo Barrio Lera; Maria Gabriela Valle Gottlieb; Carla Helena Augustin Schwanke; Maria Izabel Ugalde Marques da Rocha; Maria Fernanda Manica-Cattani; Greice Feyl dos Santos; Ivana Beatrice Manica da Cruz Journal: Mol Cell Biochem Date: 2009-03-05 Impact factor: 3.396