Literature DB >> 12183283

Development of a yeast assay for rapid screening of inhibitors of human-derived Pneumocystis carinii dihydrofolate reductase.

Liang Ma1, Qiuyao Jia, Joseph A Kovacs.   

Abstract

Human-derived Pneumocystis carinii dihydrofolate reductase (DHFR) was expressed in a Saccharomyces cerevisiae strain whose growth depends on complementation by this enzyme. We utilized a quantitative assay to measure the sensitivity of this yeast strain to DHFR inhibitors. This assay should be useful for identifying new inhibitors of human-derived P. carinii DHFR.

Entities:  

Mesh：

Substances：

Year:  2002        PMID： 12183283      PMCID： PMC127411          DOI： 10.1128/AAC.46.9.3101-3103.2002

Source DB:  PubMed          Journal:  Antimicrob Agents Chemother        ISSN： 0066-4804            Impact factor:   5.191


  14 in total

1.  Yeast as a model system to study drugs effective against apicomplexan proteins.

Authors:  C H Sibley; V H Brophy; S Cheesman; K L Hamilton; E G Hankins; J M Wooden; B Kilbey
Journal:  Methods       Date:  1997-10       Impact factor: 3.608

2.  Identification of Cryptosporidium parvum dihydrofolate reductase inhibitors by complementation in Saccharomyces cerevisiae.

Authors:  V H Brophy; J Vasquez; R G Nelson; J R Forney; A Rosowsky; C H Sibley
Journal:  Antimicrob Agents Chemother       Date:  2000-04       Impact factor: 5.191

3.  Genetic divergence of the dihydrofolate reductase and dihydropteroate synthase genes in Pneumocystis carinii from 7 different host species.

Authors:  L Ma; H Imamichi; A Sukura; J A Kovacs
Journal:  J Infect Dis       Date:  2001-10-10       Impact factor: 5.226

4.  Efficacies of lipophilic inhibitors of dihydrofolate reductase against parasitic protozoa.

Authors:  H Lau; J T Ferlan; V H Brophy; A Rosowsky; C H Sibley
Journal:  Antimicrob Agents Chemother       Date:  2001-01       Impact factor: 5.191

5.  Expression and characterization of recombinant human-derived Pneumocystis carinii dihydrofolate reductase.

Authors:  L Ma; J A Kovacs
Journal:  Antimicrob Agents Chemother       Date:  2000-11       Impact factor: 5.191

6.  Comparison of ternary complexes of Pneumocystis carinii and wild-type human dihydrofolate reductase with coenzyme NADPH and a novel classical antitumor furo[2,3-d]pyrimidine antifolate.

Authors:  V Cody; N Galitsky; J R Luft; W Pangborn; A Gangjee; R Devraj; S F Queener; R L Blakley
Journal:  Acta Crystallogr D Biol Crystallogr       Date:  1997-11-01

7.  The phenotype of a dihydrofolate reductase mutant of Saccharomyces cerevisiae.

Authors:  T Huang; B J Barclay; T I Kalman; R C von Borstel; P J Hastings
Journal:  Gene       Date:  1992-11-02       Impact factor: 3.688

8.  Inhibitors of folic acid synthesis in the treatment of experimental Pneumocystis carinii pneumonia.

Authors:  P D Walzer; C K Kim; J M Foy; M J Linke; M T Cushion
Journal:  Antimicrob Agents Chemother       Date:  1988-01       Impact factor: 5.191

9.  Potent in vitro and in vivo antitoxoplasma activity of the lipid-soluble antifolate trimetrexate.

Authors:  C J Allegra; J A Kovacs; J C Drake; J C Swan; B A Chabner; H Masur
Journal:  J Clin Invest       Date:  1987-02       Impact factor: 14.808

10.  Activity of antifolates against Pneumocystis carinii dihydrofolate reductase and identification of a potent new agent.

Authors:  C J Allegra; J A Kovacs; J C Drake; J C Swan; B A Chabner; H Masur
Journal:  J Exp Med       Date:  1987-03-01       Impact factor: 14.307
View more
  4 in total

1.  Mutations of Pneumocystis jirovecii dihydrofolate reductase associated with failure of prophylaxis.

Authors:  Aimable Nahimana; Meja Rabodonirina; Jacques Bille; Patrick Francioli; Philippe M Hauser
Journal:  Antimicrob Agents Chemother       Date:  2004-11       Impact factor: 5.191

2.  Evaluation of the activities of pyrimethamine analogs against Plasmodium vivax and Plasmodium falciparum dihydrofolate reductase-thymidylate synthase using in vitro enzyme inhibition and bacterial complementation assays.

Authors:  Sasinee Bunyarataphan; Ubolsree Leartsakulpanich; Supannee Taweechai; Bongkoch Tarnchompoo; Sumalee Kamchonwongpaisan; Yongyuth Yuthavong
Journal:  Antimicrob Agents Chemother       Date:  2006-09-05       Impact factor: 5.191

Review 3.  A Molecular Window into the Biology and Epidemiology of Pneumocystis spp.

Authors:  Liang Ma; Ousmane H Cissé; Joseph A Kovacs
Journal:  Clin Microbiol Rev       Date:  2018-06-13       Impact factor: 26.132

4.  Lausannevirus Encodes a Functional Dihydrofolate Reductase Susceptible to Proguanil.

Authors:  L Mueller; P M Hauser; F Gauye; G Greub
Journal:  Antimicrob Agents Chemother       Date:  2017-03-24       Impact factor: 5.191
  4 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.