PURPOSE: The aim of this investigation was to describe the alterations in oral mucosa after radiotherapy. METHODS AND MATERIALS: Biopsies were taken from patients before irradiation, at 60 Gy, and 6-12 months after radiotherapy. Histomorphological evaluation of the vessels was performed, and endothelial expression of ICAM-1, VCAM-1, and E-selectin was also evaluated, as well as distribution of LFA-1-, Mac-1-, VLA-4-, RM3/1-, 27E10-, and 25F9-bearing cells in the subepithelial tissue. RESULTS: The expression of ICAM-1 was downregulated after radiotherapy, whereas the percentage of LFA-1- and VLA-4-bearing cells increased. VCAM-1 remained at low levels. The subepithelial infiltration was still dominated by RM3/1-positive macrophages. The number of vessels decreased, while the lumina of the remaining vessels in the deeper connective layer increased. CONCLUSIONS: The late effects of radiotherapy are characterized by a decreased number of blood vessels and by significantly different expression patterns of the adhesion molecules studied, and of integrins and macrophage subpopulations compared to the conditions before irradiation and at 60 Gy.
PURPOSE: The aim of this investigation was to describe the alterations in oral mucosa after radiotherapy. METHODS AND MATERIALS: Biopsies were taken from patients before irradiation, at 60 Gy, and 6-12 months after radiotherapy. Histomorphological evaluation of the vessels was performed, and endothelial expression of ICAM-1, VCAM-1, and E-selectin was also evaluated, as well as distribution of LFA-1-, Mac-1-, VLA-4-, RM3/1-, 27E10-, and 25F9-bearing cells in the subepithelial tissue. RESULTS: The expression of ICAM-1 was downregulated after radiotherapy, whereas the percentage of LFA-1- and VLA-4-bearing cells increased. VCAM-1 remained at low levels. The subepithelial infiltration was still dominated by RM3/1-positive macrophages. The number of vessels decreased, while the lumina of the remaining vessels in the deeper connective layer increased. CONCLUSIONS: The late effects of radiotherapy are characterized by a decreased number of blood vessels and by significantly different expression patterns of the adhesion molecules studied, and of integrins and macrophage subpopulations compared to the conditions before irradiation and at 60 Gy.
Authors: Martin Scheer; Juliana Fuss; Mehmet Ali Derman; Matthias Kreppel; Jörg Neugebauer; Daniel Rothamel; Uta Drebber; Joachim E Zoeller Journal: Oral Maxillofac Surg Date: 2015-08-13
Authors: Renée Helmers; Dan M J Milstein; Nina F Straat; Arash Navran; David N Teguh; Robert A van Hulst; Ludi E Smeele; Jan de Lange Journal: Head Neck Date: 2022-04-29 Impact factor: 3.821
Authors: Mette Marcussen; Mads Sønderkær; Julie Støve Bødker; Maria Andersen; Søren Nielsen; Charles Vesteghem; Ilse Christiansen; Olav Jonas Bergmann; Martin Bøgsted; Karen Dybkær; Mogens Vyberg; Hans Erik Johnsen Journal: PLoS One Date: 2018-01-16 Impact factor: 3.240