| Literature DB >> 12182937 |
Dawn M Stanek1, Jan Pohl, Joe W Crim, Mark R Brown.
Abstract
A neuropeptide F (NPF) was isolated from an extract of adult Aedes aegypti mosquitoes based on its immunoreactivity in a radioimmunoassay for Drosophila NPF. After sequencing the peptide, cDNAs encoding the NPF were identified from head and midgut. These cDNAs encode a prepropeptide containing a 36 amino acid peptide with an amidated carboxyl terminus, and its sequence shows it to be a member of the neuropeptide F/Y superfamily. Immunocytochemistry and Northern blots confirmed that both the brain and midgut of females are likely sources of NPF, found at its highest hemolymph titer before and 24 h after a blood meal. Copyright 2002 Elsevier Science Inc.Entities:
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Year: 2002 PMID: 12182937 DOI: 10.1016/s0196-9781(02)00074-8
Source DB: PubMed Journal: Peptides ISSN: 0196-9781 Impact factor: 3.750