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Literature DB >> 1218095

The reaction of choline dehydrogenase with some electron acceptors.

Abstract

1. The choline dehydrogenase (EC 1.1.99.1) WAS SOLUBILIZED FROM ACETONE-DRIED POWDERS OF RAT LIVER MITOCHONDRIA BY TREATMENT WITH Naja naja venom. 2. The kinetics of the reaction of enzyme with phenazine methosulphate and ubiquinone-2 as electron acceptors were investigated. 3. With both electron acceptors the reaction mechanism appears to involve a free, modified-enzyme intermediate. 4. With some electron acceptors the maximum velocity of the reaction is independent of the nature of the acceptor. With phenazine methosulphate and ubiquinone-2 as acceptors the Km value for choline is also independent of the nature of the acceptor molecule. 5. The mechanism of the Triton X-100-solubilized enzyme is apparently the smae as that for the snake venom solubilized enzyme.

Entities: Chemical Species

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Year: 1975 PMID： 1218095 PMCID： PMC1172417 DOI： 10.1042/bj1510677

Source DB: PubMed Journal: Biochem J ISSN： 0264-6021 Impact factor: 3.857

15 in total

The reaction of choline dehydrogenase with some electron acceptors.

1. THE MOLAR EXTINCTION COEFFICIENT OF 2,6-DICHLOROPHENOL INDOPHENOL.

2. Studies on choline dehydrogenase. I. Extraction in soluble form, assay, and some properties of the enzyme.

3. Intermediates in the catalytic action of lipoyl dehydrogenase (diaphorase).

4. Studies on succinic dehydrogenase. I. Preparation and assay of the soluble dehydrogenase.

5. Preparation and some properties of soluble choline dehydrogenase.

6. A micro biuret method for protein determination; determination of total protein in cerebrospinal fluid.

7. The reduction of ubiquinone in rat liver mitochondria associated with the oxidation of choline.

8. Essentiality of coenzyme Q for the oxidation of -glycerophosphate by pig brain mitochondria.

9. Essentiality of ubiquinone for choline oxidation in rat liver mitochondria.

10. Preparation and some properties of L-3-glycerophosphate dehydrogenase from pig brain mitochondria.

1. Solubilization, partial purification and properties of N-methylglutamate dehydrogenase from Pseudomonas aminovorans.

Review 2. Human choline dehydrogenase: medical promises and biochemical challenges.

Review 3. Metabolic Targets of Coenzyme Q10 in Mitochondria.

Review 4. The Roles of Coenzyme Q in Disease: Direct and Indirect Involvement in Cellular Functions.