OBJECTIVE: We recently reported that transcription of human endogenous retrovirus (HERV) clone 4-1-like sequences is increased in patients with systemic lupus erythematosus (SLE). We therefore investigated the role of DNA methylation in the transcription of this HERV. METHODS: The effect of a demethylating agent, 5-aza-deoxycytidine (5-aza C), on the transcription of HERV clone 4-1 in healthy individuals and patients with SLE was examined using reverse transcriptase-PCR and real-time quantitative PCR. RESULTS: 5-aza C increased clone 4-1-like messenger RNA in healthy controls, but not in patients with SLE. CONCLUSION: Defects of methylation may contribute to the transcription of HERV in patients with SLE and this may be related to the pathogenesis of SLE.
OBJECTIVE: We recently reported that transcription of human endogenous retrovirus (HERV) clone 4-1-like sequences is increased in patients with systemic lupus erythematosus (SLE). We therefore investigated the role of DNA methylation in the transcription of this HERV. METHODS: The effect of a demethylating agent, 5-aza-deoxycytidine (5-aza C), on the transcription of HERV clone 4-1 in healthy individuals and patients with SLE was examined using reverse transcriptase-PCR and real-time quantitative PCR. RESULTS:5-aza C increased clone 4-1-like messenger RNA in healthy controls, but not in patients with SLE. CONCLUSION: Defects of methylation may contribute to the transcription of HERV in patients with SLE and this may be related to the pathogenesis of SLE.
Authors: Katherine B Chiappinelli; Pamela L Strissel; Alexis Desrichard; Huili Li; Christine Henke; Benjamin Akman; Alexander Hein; Neal S Rote; Leslie M Cope; Alexandra Snyder; Vladimir Makarov; Sadna Budhu; Sadna Buhu; Dennis J Slamon; Jedd D Wolchok; Drew M Pardoll; Matthias W Beckmann; Cynthia A Zahnow; Taha Merghoub; Taha Mergoub; Timothy A Chan; Stephen B Baylin; Reiner Strick Journal: Cell Date: 2015-08-27 Impact factor: 41.582
Authors: Christof C Smith; Kathryn E Beckermann; Dante S Bortone; Aguirre A De Cubas; Lisa M Bixby; Samuel J Lee; Anshuman Panda; Shridar Ganesan; Gyan Bhanot; Eric M Wallen; Matthew I Milowsky; William Y Kim; W Kimryn Rathmell; Ronald Swanstrom; Joel S Parker; Jonathan S Serody; Sara R Selitsky; Benjamin G Vincent Journal: J Clin Invest Date: 2018-10-02 Impact factor: 14.808
Authors: R Takahashi; A Tsutsumi; K Ohtani; D Goto; I Matsumoto; S Ito; N Wakamiya; T Sumida Journal: Clin Exp Immunol Date: 2004-06 Impact factor: 4.330