OBJECTIVE: To investigate the level of interleukin-6 (IL-6) expression in the synovial-like interface membrane (SLIM) and in the pseudosynovial tissue surrounding the artificial hip joints, as well as in the pseudosynovial fluid from aseptically loosened total hip replacement (THR) prostheses. METHODS: A series of methods were used in this study including immunohistochemical staining, double immunofluorescence labeling, enzyme-linked immunosorbent assay (ELISA), and reverse transcriptase polymerase chain reaction (RT-PCR). RESULTS: IL-6 was found in all SLIM and the pseudosynovial tissue samples from aseptic loosening of THR. Semi-quantitative morphometry showed that IL-6 containing cells were more numerous in the SLIM (911 +/- 197; p < 0.01) and the pseudosynovial tissue samples (883 +/- 310; p < 0.01) than in the control synovial tissue (291 +/- 184). Double labeling confirmed that macrophages and fibroblasts were the predominant cell types expressing IL-6. These findings were confirmed by RT-PCR. ELISA revealed no difference in the IL-6 concentration between the pseudosynovial fluid and the control synovial fluid obtained from the patients undergoing hip arthroscopy. CONCLUSIONS: IL-6 locally produced in SLIM may in a paracrine manner contribute to periprosthetic osteolysis of the nearby bone. In contrast, fluid phase IL-6 does not seem to contribute to this end.
OBJECTIVE: To investigate the level of interleukin-6 (IL-6) expression in the synovial-like interface membrane (SLIM) and in the pseudosynovial tissue surrounding the artificial hip joints, as well as in the pseudosynovial fluid from aseptically loosened total hip replacement (THR) prostheses. METHODS: A series of methods were used in this study including immunohistochemical staining, double immunofluorescence labeling, enzyme-linked immunosorbent assay (ELISA), and reverse transcriptase polymerase chain reaction (RT-PCR). RESULTS:IL-6 was found in all SLIM and the pseudosynovial tissue samples from aseptic loosening of THR. Semi-quantitative morphometry showed that IL-6 containing cells were more numerous in the SLIM (911 +/- 197; p < 0.01) and the pseudosynovial tissue samples (883 +/- 310; p < 0.01) than in the control synovial tissue (291 +/- 184). Double labeling confirmed that macrophages and fibroblasts were the predominant cell types expressing IL-6. These findings were confirmed by RT-PCR. ELISA revealed no difference in the IL-6 concentration between the pseudosynovial fluid and the control synovial fluid obtained from the patients undergoing hip arthroscopy. CONCLUSIONS:IL-6 locally produced in SLIM may in a paracrine manner contribute to periprosthetic osteolysis of the nearby bone. In contrast, fluid phase IL-6 does not seem to contribute to this end.
Authors: Vinod Dasa; Jill M Kramer; Sarah L Gaffen; Keith L Kirkwood; William M Mihalko Journal: Clin Orthop Relat Res Date: 2012-07 Impact factor: 4.176
Authors: Arihiko Kanaji; Marco S Caicedo; Amarjit S Virdi; D Rick Sumner; Nadim J Hallab; Kotaro Sena Journal: Bone Date: 2009-06-02 Impact factor: 4.398
Authors: S B Goodman; E Gibon; J Pajarinen; T-H Lin; M Keeney; P-G Ren; C Nich; Z Yao; K Egashira; F Yang; Y T Konttinen Journal: J R Soc Interface Date: 2014-01-29 Impact factor: 4.118
Authors: Michael Darowish; Ra'Kerry Rahman; Ping Li; Susan V Bukata; Jill Gelinas; Willis Huang; Lisa M Flick; Edward M Schwarz; Regis J O'Keefe Journal: Bone Date: 2009-06-12 Impact factor: 4.398
Authors: Antony K Sorial; Sami A Anjum; Michael J Cook; Tim N Board; Terence W O'Neill Journal: Ther Adv Musculoskelet Dis Date: 2020-12-16 Impact factor: 5.346