MATERIALS AND METHODS: Recombinant high density lipoprotein (rHDL) particles were prepared with defined composition (phosphatidylcholine, apolipoprotein A-1, cholesterol and cholesteryl esters) and a molecular weight of approximately 187,000 kdaltons. Three molecules of taxol per rHDL particle were incorporated into these rHDL complexes. RESULTS: Cholesteryl ester and taxol (HDL core components,) were taken up efficiently by several cancer cell lines compared to transformed normal ovarian cells (HGL5) used as the control. Immunoblotting with the scavenger receptor (SR-B1) antibody revealed strong immunoreactivity of several cancer cell lines. CONCLUSION: Our studies indicated that rHDL complexes can be used as efficient drug delivery vehicles due to the ability of cancer cells to acquire HDL core components. Targeting of anticancer drugs as components of the rHDL complex could result in reduction of toxic side-effects during chemotherapy.
MATERIALS AND METHODS: Recombinant high density lipoprotein (rHDL) particles were prepared with defined composition (phosphatidylcholine, apolipoprotein A-1, cholesterol and cholesteryl esters) and a molecular weight of approximately 187,000 kdaltons. Three molecules of taxol per rHDL particle were incorporated into these rHDL complexes. RESULTS:Cholesteryl ester and taxol (HDL core components,) were taken up efficiently by several cancer cell lines compared to transformed normal ovarian cells (HGL5) used as the control. Immunoblotting with the scavenger receptor (SR-B1) antibody revealed strong immunoreactivity of several cancer cell lines. CONCLUSION: Our studies indicated that rHDL complexes can be used as efficient drug delivery vehicles due to the ability of cancer cells to acquire HDL core components. Targeting of anticancer drugs as components of the rHDL complex could result in reduction of toxic side-effects during chemotherapy.
Authors: Walter J McConathy; Sulabha Paranjape; Linda Mooberry; Sabitha Buttreddy; Maya Nair; Andras G Lacko Journal: Drug Deliv Transl Res Date: 2011-04 Impact factor: 4.617
Authors: Mian M K Shahzad; Lingegowda S Mangala; Hee Dong Han; Chunhua Lu; Justin Bottsford-Miller; Masato Nishimura; Edna M Mora; Jeong-Won Lee; Rebecca L Stone; Chad V Pecot; Duangmani Thanapprapasr; Ju-Won Roh; Puja Gaur; Maya P Nair; Yun-Yong Park; Nirupama Sabnis; Michael T Deavers; Ju-Seog Lee; Lee M Ellis; Gabriel Lopez-Berestein; Walter J McConathy; Laszlo Prokai; Andras G Lacko; Anil K Sood Journal: Neoplasia Date: 2011-04 Impact factor: 5.715