AIMS: To identify a chromosomal marker with signature nucleotides specific for Bacillus anthracis. METHODS AND RESULTS: Repetitive element polymorphism-polymerase chain reaction with BOX-A1R primer was used to discriminate 52 strains of all six species of the 'B. cereus group'. A B. anthracis signature fragment, named AC-390, was cloned and sequenced. The deduced amino acid sequence was homologous to that of YwfK of B. subtilis. Using two internal primers, the AC-390 fragment was sequenced from two other B. anthracis strains as well as from strains of B. cereus and B.thuringiensis which have an AC-390 fragment homologous to that of B. anthracis as shown by Southern hybridization experiments. CONCLUSIONS: Two new signature sequences specific for B. anthracis were identified on a chromosomal fragment homologous to YwfK, a transcriptional regulator of B. subtilis. SIGNIFICANCE AND IMPACT OF THE STUDY: These results show a new chromosomal DNA trait useful for distinguishing B. anthracis from the related species of the B. cereus group, regardless of the presence of the virulence plasmids pXO1 and pXO2.
AIMS: To identify a chromosomal marker with signature nucleotides specific for Bacillus anthracis. METHODS AND RESULTS: Repetitive element polymorphism-polymerase chain reaction with BOX-A1R primer was used to discriminate 52 strains of all six species of the 'B. cereus group'. A B. anthracis signature fragment, named AC-390, was cloned and sequenced. The deduced amino acid sequence was homologous to that of YwfK of B. subtilis. Using two internal primers, the AC-390 fragment was sequenced from two other B. anthracis strains as well as from strains of B. cereus and B.thuringiensis which have an AC-390 fragment homologous to that of B. anthracis as shown by Southern hybridization experiments. CONCLUSIONS: Two new signature sequences specific for B. anthracis were identified on a chromosomal fragment homologous to YwfK, a transcriptional regulator of B. subtilis. SIGNIFICANCE AND IMPACT OF THE STUDY: These results show a new chromosomal DNA trait useful for distinguishing B. anthracis from the related species of the B. cereus group, regardless of the presence of the virulence plasmids pXO1 and pXO2.
Authors: Sergei G Bavykin; Vladimir M Mikhailovich; Vladimir M Zakharyev; Yuri P Lysov; John J Kelly; Oleg S Alferov; Igor M Gavin; Alexander V Kukhtin; Joany Jackman; David A Stahl; Darrell Chandler; Andrei D Mirzabekov Journal: Chem Biol Interact Date: 2007-09-12 Impact factor: 5.192
Authors: Ivan Magriñá; Miriam Jauset-Rubio; Mayreli Ortiz; Herbert Tomaso; Anna Simonova; Michal Hocek; Ciara K O'Sullivan Journal: ACS Omega Date: 2019-12-11